Effects Of Sleep Deprivation On The Expression Of Phosphorylated EIF2a And Phosphorylated PERK In The Rat Cerebral Cortex And Drug Intervention

To discuss the effect of PERK activation signal pathway during SD and the possible neuroprotective effect of edaravone by observing the changes of PERK activation and eIF2α(p) expression in the rat cerebral cortex on different stages of rapid eye movement sleep deprivation and effects of edaravone. The modified multiple platform methods(MMPM)were established as the rapid eye movement(REM)sleep deprivation method.Using immunohistochemistry,the eIF2α(p)staining was showed in cortex neurons cytoplasmic localization. The western blot results showed that the expression of eIF2α(p)and PERK(p) in cerebral cortex was increased after sleep deprivation . The levels of eIF2α(p)and PERK(p) showed a trend from increase to decline. The administration of edaravone noticeably reduced the levels of PERK(p)and eIF2α(p). Short-time REM sleep deprivation does induce the phosphorylation of eIF2αin the rat's cerebral cortex, which cause translation inhibition. Translation inhibition regulation is one of effective mechanisms to maintain the organisms'normal function. The activation of eIF2αkinase PERK in the rat's frontal cortex after the several hours of REM sleep deprivation suggest that sleep deprivation initiated the phosphorylation of eIF2αpathway during UPR in the ER stress. It possibly is one of brain damage mechanisms. edaravone can protect against endoplasmic reticulum dysfunction in the cerebral cortex induced sleep deprivation.