Preparation Of Ginseng Pectin-Doxorubicin Complex And Studies On Its Anti-cancer Activity

Abstract Doxorubicin (DXR), a well known anti-cancer drug, is a member of the anthracycline ring antibiotics with abroad spectrum of anti-tumor activity. However, it produces side effects such as myelosuppression, cardiotoxicity and other toxicities.To decrease toxicity and increase the effectiveness against cancer, DXR has been connected with ginseng pectin through a short peptide chain. DXR is coupled to a peptide to give a DXR-peptide complex, it is delivered to tumor tissue through the affinity interaction of ginseng pectin and malignant cell. The linker short peptide of ginseng pectin and DXR is hydrolyzed by elastase to give a delayed release of DXR.In this thesis six ginseng pectin-DXR complexes were synthesized. The linkers used to connecte DXR to ginseng pectin were one amino acid, dipeptide and tripeptide, respectively. Ginseng pectin used in test was consisted ofα-(1→4)-D-GalUA (galactose-uronic acid). The complexes and intermediates in the research work were characterized with 1H NMR, 13C NMR and UV. In the complexes, the DXR content was from 0.78% to 1.61%. The inhabitation ratio of L929 and H7402 of one ginseng pectin-DXR complex is better than that of DXR itself, but all of the six complexes are better than ginseng pectin.