| Objective: To study the expression of FADD and caspase-3 protein and the effects of after galactose-cerebroside in brain tissue following transient cerebral ischemic reperfusion in rats.Methods: The middle cerebral artery occlusion(MCAO) models were established by using the intraluminal suture occlusion method in SD rats. Thirty-six male SD rats were divided randomly into: three groups: sham-operated group, cerebral ischemia/reperfusion group and galactose-cerebroside therapic group. The expressions of FADD, caspase-3 in brain tissue were detected by immunohistochemistry technique at sixth hour,1st day,3rd day,7th day after ischemic reperfusion(IR). Pathologic changes were evaluated by Hematoxylin and eosin stain.; Expression results were comparatively analyzed with statistic soft package of Spss11.0.Results: Experimental results revealed that FADD and caspase-3 protein were expressed in neurons and gliaeytes of brain tissue in 36 rats. Compared with sham operation group, the expression of FADD and caspase-3 protein in neurons and gliacytes of ischemia-reperfusion rats were evidently increased at the sixth hour,1st day,3rd day. 7th day(P＜0.01). Compared with cerebral ischemia-reperfusion group, the expression of FADD and caspase-3 protein in neurons and gliacytes of rats in galactose-cerebroside treatment group were evidently decreased at the sixth hour,1st day,3rd day,7th day (P＜0.01)Conclusions:1. Expressions of FADD and caspase-3 protein in neurons and gliacytes of rats were evidently increased following cerebral ischemic reperfusion, indicating FADD, caspase-3 maybe involved in apoptosis induced by cerebral ischemia reperfusion injury.2. Their expression in neurons and gliacytes of rats in galactose-cerebroside treatment group were evidently decreased, the brain tissue injury was alleviated, demon- strating the protective effect of galactose-cerebroside maybe related with intervencing the expression of FADD and caspase-3.
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