The Expression Of Cardiomyocytes SP .CGRP Induced By Norepinepherine, Cardiomyocytes Apoptosis And The Intervention Effects Of SP To It

Objective To investigate the expression of cardiomyocytes SP,CGRP induced by norepinepherine,cardiomyocytes apoptosis and the intervention effects of SP to it.Methods(1)The model of original cultured cardiomyocytes of SD rat was established and to observe and identify neonatal rat cardiomyocytes with morphology and immunocytochemical assay method under a reverse microscope.8 holes rat cardiomyocytes subfused after 4-5 days were randomly divided 2 groups,4 holes in each group.Group 1 is negative control group.Group 2 is experimental group.(2)20 holes rat cardiomyocytes were randomly divided into 5 groups,4 holes in each group: The control group,1h group,3h group,6h group,12h group.Test the samples after NE(10^-8 mol·L^-1)was added to group2 to group5,observe the expression of cardiomyocytes SP,CGRP induced by NE through IC approach.(3)16 holes rat cardiomyocytes were randomly divided into 4 groups,4 holes in each group: The control group,1h group,3h group,6h group.Test the samples after NE(10^-8mol·L^-1)was added to group2 to group5.Observe the cardiomyocytes apoptosis induced by NE through tunel approach.(4)12 holes rat cardiomyocytes were randomly divided into 3 groups,4 holes in each group: the control group;NE group;SP+NE group;add 10^-8mol·L^-1NE to each group to induce,at the same time,add SP(10Smol·L^-1)to group 3 for lh.Test the samples by Flow Cytometry(FCM) technique and observe the effect of SP on NE-induced cardiomyocytes_apoptosis. Results(1)Compared with the control group,the expression of cardiomyocytes SP,CGRP in each group(1h,3h,6b,12h)were significantly elevated(p＜0.05)with the inducing of NE and peaked in the group induced by 10^-8mol·L^-1NE 6 hours later.(2)Compared with the control group,NE10^-8mol·L^-1can significantly induce cardiomyocytes apoptosis for 1h,3h,and 6h.(p＜0.05)and the most significant at the point of 1b.(3)The cardiomyocytes apoptosis rate is significantly lower in SP+NE group than in 1h group(NE10^-8mol·L),(P＜0.05). Conlusions(1)NE can induce the elevating of cardiomyocytes_SP,CGRP expression.(2)NE can induce cardiomyocytes apoptosis.(3)SP preintervention can siginificantly inhibit the cardiomyocytes apoptosis induced by NE,which shows that SP may protect cardiomuscular at the cell level.