| Objective:To study the effects of anti-inflammation of the four traditional Chinese prescriptionson experimental rats and provide experimental basis for clinical use,we observed the effectsof anti-inflammation and investigated the effects of the four traditional Chinese prescriptions tothe inflamed PMN by the method of serum pharmacology.Method: Experiment 1. The inhibition of the four traditional Chinese prescriptions on1％carrageenin-induced foot-swelling rats: 153 clean Wistar rats were divided into 15groups, namely normal group, model group, cortisol group, Jinlingzi powder groups oflow, middle, high dose, Tongxie prescription groups of low, middle, high dose, Shaogan decoctiongroups of low, middle, high dose, Chaiyu decoction groups of low, middle, high dose. On day1, rats were treated with weighting, numbering,and grouping. On day 2, 3, rats were measured theright foot volume in normal condition with improved volume measurer. On day 4, each groupwas administered corresponding drug in the morning. on day 5 to 8, treatment was the same asday 4. On day 9, after administered corresponding drug, rats were injected subcutaneously with1ml syringe except the normal group, the right foot swellon of rats were measured beforeinjection and after injection 0.5h, 1h, 2h, 4h, 6h.Experiment 2. To observed the inhibition of the four traditional Chinese prescriptions on crotonoil mixed inflamed agent induced-ear swelling mice: 150 clean KunMing mice were divided into15 groups, namely model group,cortisol group, aspirin group, Jinlingzi powder groups oflow, middle, high dose, Tongxie prescription groups of low, middle, high dose, Shaogan decoctiongroups of low, middle, high dose, Chaiyu decoction groups of low, middle, high dose. On day1, mice were treated with weighting, numbering, and grouping, model group was administeredwater, and other groups were administered corresponding drugs at afternoon. On day 2 to5,treatment was the same as day 1. After 1h, the both sides of mice right ear were painted with 2％croton oil mixed inflamed agent 50μl, the left ear were not treated. After 4h, the right ear swellonof mice were measured.Experiment 3. To observed the inhibition of the four traditional Chinese prescriptions on1％carrageenin-induced inflammatory medium of inflammatory liquid in rat airbag: 150 cleanWistar rats were divided into 15 groups, namely normal group, model group, cortisolgroup, Jinlingzi powder groups of low, middle, high dose, Tongxie prescription groups oflow, middle, high dose, Shaogan decoction groups of low, middle, high dose, Chaiyu decoctiongroups of low, middle,high dose.On day 1,rats were treated with weighting,numbering,andgrouping.On day 2,3,the back of rats were haired shaved, then were injected subcutaneously air20ml (by filtering)and made airbag. On day 4, normal group and model group were administered water, and other groups were administered corresponding drugs. On day 4 and day 8,, the back ofrats were injected subcutaneously air 10ml to keep the airbag, On day 5 to 9, treatment was thesame as day 4. On day 9, the airbags of rats were injected 1％carrageenin 2ml for each oneexcept normal group. After 4h, all rats were withdrawed blood from inferior vena cava andinflammatory liquid. Measure the content of PGE2, NO, IL-6 in the inflammatory liquid.Experiment 4. the inhibition of serum the four traditional chinese prescriptions prepared toPMN: 1％carrageenin lml were injected into pleural of rats. After 4h, rats were killed andwashed pleural with cold 0.9％NaC1 solution 5ml twice, collect PMN, count the leucocytes, thePMN were centrifuged at 900g, 5min, the supematant were removed, erythrocytes were dissolvedby 0.87％Nt-hC1 5ml, PMNs were left. PMNs were washed with PBS twice and resuspended. ThePMNs were cultured with RPIM 1640 culture medium for one night,then washed with RPIM1640 culture medium, centrifuged at1500 r/min, the supematant were removed, and the cells werequantified with 6ml RPIM 1640 culture medium, resuspended to end concentration 3×105/ml.3×105/ml PMNs were inoculated onto two 96 hole plates and 100μl per hole. the PMNs weredivided into culture medium group,PBS group, normal group, Jinlingzi powder group, Tongxieprescription group, Shaogan prescription group,Chaiyu decoction group,Tetrahydropalmatinestandard(10-5, 10-4, 10-3), paeoniflorin standard(10-5, 10-4, 10-3), curcumin standard(10-5, 10-4, 10-3), cortisol group, blank group .Blank group was entered 150gl RPIM 1640, cortisolgroup was entered 50μl cortisol and other groups were entered 50μl serum or PBS or standardsolution per hole. 37℃5％CO2 incubation. While measuring, each hole were entered 20μlluminol, then 150μl Zymosan,and measured luminescent with luminiferous instrument.Result: Experiment 1. The inhibition of four traditional Chinese prescriptions is strongremarkably at 4h.Experiment 2. The result indicate the Jinlingzi powder groups of low, middle, high dose,Shaogan decoction groups of middle,high dose all inhibit remarkably the ear swellon that thecroton oil mixed inflamed agent induced,but the effect is not dose-dependent, the inhibition ofTongxie prescription and Chaiyu decoction is obvious weak.Experiment 3. The inhibition of Jinlingzi powder and Shaogan prescription is remarkable toPGE2 in inflammatory liquid of rat airbag. The inhibition of Jinlingzi powder, Tongxieprescription, Shaogan decoction, Chaiyu decoction is remarkable to IL-6 in inflammatory liquidof rat airbag. The inhibition of Jinlingzi powder, Tongxie prescription, Shaogandecoction, Chaiyu decoction is remarkable to NO in inflammatory liquid of rat airbag.Experiment 4. Normal serum and drug-contained serum were prepared successfully. Theinhibition of Jinlingzi powder-contained serum, Tongxie prescription -contained serum, Shaogandecoction- contained serum is remarkable to inflamed PMNs by chemiluminescence method.Conclusion: 1. Jinlingzi powder, Tongxie prescription,Shaogan decoction, Chaiyu decoction haveobvious anti-inflammation effects in rats internally and externally. 2. The effects of Jinlingzi powder, Tongxie prescription, Shaogan decoction on foot-swellon arethe same as cortisol ; The effects of ear-swellon are the same as aspirin.3. The mechanism of anti-inflammation is partially to inhibit prouding of PGEE, NO, IL-6. Theeffects of PGE2, NO, IL-6 are the same as cortisol.4. The mechanism of Shaogan decoction is to influence hypothalamo- pituitary- adrencortical.
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