Suppressive Effects Of Dog Bile On CCl₄-induced Hepatic Fibrosis In Rats

Objective To investigate the suppressive effects of dog bile on CCl4 induced hepatic fibrosis in rats.Methods The rats were divided in to five groups, Hepatic fibrosis models were induced by intraperitoneal injection of 50% CCl4 for 12wks in rats. At the same time, rats in treatment groups were given bear bile and dog bile (high and low dosages) solution by gastric garage once a daily for 12wk. At the end of 12th wk, all the rats were sacrificed and specimens of serum and liver tissue were collected for the studies. Liver/body weight ratio and serum liver enzymes were determined. H-E staining was used to observe the pathologic changes. Degrees of fibrosis and area of collageneous fibers in liver were determined by direct red staining .The Kupffer cells positive for EDl and hepatic stellate cells (HSCs) positive for alpha-smmoth muscle actin (α-SMA) in the liver were determined by immunohistochemistry.Results Compared with the model group, the liver/body weight ratio was significantly increased (P＜0.001), the levels of serum AST and ALT were significantly decreased ,and the TP content was increased in dog bile groups. Histopathological observation showed that the Dog bile has better suppressive liver fibrosis effects. The areas of collageneous fibers were significantly decreased(P＜0.01), and the number of ED1 andα—SMA-positive cells were markedly reduced in dog bile groups.Conclusion Dog bile may suppress on CCl4 induced hepatic fibrosis probably via its hepato-protective and anti-inflammation effects.