| Soy food, a traditional kind of food in human beings' lives, has been associated with a contribution of detoxification and hepaticprotenction, which are related to the biological transformation and metabolism activation of soybean isoflavone. The purpose of this study is to investigate genistein's influence on the association between the activities of ALI,AST,UGTs and liver lesions caused by APAP; and is to explore every single difference of UGT1A and UGT2B families mRNA expression during acetaminophen hepatic toxicity.As it is turned out:(1)The mice experiment revealed that the activities of ALT and AST, the contents of GSH and APAP and the histological situation were significantly different among dose group. In all dose groups, genistein significantly decreased the activities of ALT and AST, increased the contents of GSH and APAP, and gradually improved the pathological changes in mice's livers after acetaminophen exposure.(2) These activities of 4-MU UGT and 4-NP UGT were significantly decreased after acetaminophen exposure; whereas high dose of genistein was capable to increase these activities of 4-MU UGT and 4-NP UGT. The regulation of UDP-glucuronosyltransferases (UGTs) by genistein in acetaminophen hepatic toxicity is not well characterized. To determine the response of hepatic UGTs during this, mice were administered various doses of genistein. As a result, compared with normal control group, hepatic mRNA expression of UGT1A1,UGTIA10,UGT2B34,UGT2B35,UGT2B36,UGT2B37,and UGT2B38 were similarly down-regulated after exposure, whereas UGT1A6,UGTIA^ UGT2B1 and UGT2B5 mRNA expression were unchanged. Furthermore, hepatic mRNA expression of UGTIA9,UGT1A10 and UGT2B 36 were similarly up-regulated after genistein exposure. The results indicated that genistein could induce hepatic mRNA expression of UGT1A9,UGT1A10 and UGT2B 36 in mice liver. It is hopeful that this discovery may help to explain the clearance mechanism of genistein to exogenous in molecular level.
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