The Active Change Of Glyceraldehyde-3-phosphate Dehydrogenase In The Microvascular Complications Of Diabetes Before And After Puerarin Treatment

Background & Objective:Nowadays, the microvascular complication of diabetes mellitus is one of the main reasons which cause the diabetic to be disabled or death. Research shows that hyperglycemia-induced mitochondrial electron transport chain superoxide dismutase overproduction may be diabetic microangiopathy major Pathogenesis. Puerarin is a commonly found in the plant kingdom which has a clear antioxidant substance. For this study, we observed puerarin treatment of diabetic microangiopathy in patients, Compared GAPDH, Oxidative stress indicators, as well as general indicators of change, the certification of diabetes oxidative stress in existence, GAPDH explores the different state of oxidative stress and oxidative activity indicators.Research Methods:1. Experiment grouping using a case-control study, were included in type 2 diabetic microangiopathy in 80 patients, were divided into puerarin treatment group and the conventional treatment group, normal control group (N) 67. 2. Experimental intervention group N and treatment of all patients with diabetic microangiopathy in the early morning are fasting to test serum maleic dialdehyde (MDA), Glutathione (GSH) content, superoxide dismutase (SOD), Glutathione peroxidase (GSH - PX), blood GAPDH activity, and fasting and after eating 2-hours postprandial blood glucose, glycated hemoglobin (HbA1c), Urinary albumin excretion rate (UAER). Puerarin conventional treatment group treated diabetes with Puerarin injection (Trade Name: Buruining) 400mg to 100ml saline infusion, day 1, 2 weeks for a course of treatment. After one course of treatment, all of diabetes was measured these indexes mentioned above. Conventional treatment group use conventional diabetes treatment method after two weeks to review each index above. Meanwhile it should be observed blood, urine, liver and kidney function, blood pressure and ECG changes and record adverse events.3. Measurement index and blood glucose method using Roche vitality-glucose measured; urinary albumin excretory rate Determination: gold standard Determination (endocrine experimentation room of the first affiliated hospital of zhengzhou university); HbA1c Determination: Using chromatography (endocrine experimentation room of the first affiliated hospital of zhengzhou university); Determination of serum concentration of MDA : using thiobarbituric acid assay; SOD and GSH Determination: using determination of the chemical colorimetry; Determination of GSH - PX : using phenol couplin determination. Detection Kits: both the product of the Biotechnology Research Institute of Nanjing JianCheng. GAPDH Determination: spectrophotometry.4. Statistical analysis using SPSS10.0for Windows statistical analysis software, the data is presented as mean±standard deviation. Statistically before and after treatment were compared using paired t-test. Normal control group and two patients with several samples t-test and the indexes used Pearson correlation analysis, A value of P <0.05 was statistically significant.Result: 1. Glucose, glycated hemoglobin and urine albumin excretion rate results Compared with N group, patients with diabetic microvascular fasting plasma glucose (FPG), after eating 2-hour postprandial blood glucose (2hPG). glycosylated hemoglobin (HbA1c), urinary albumin excretion rate (UAER) significantly increased, a significant statistically difference; Puerarin treatment group compared with those before treatment, patient's FPG,2hPG,UAER are significantly lower, a significant statistically difference. Conventional treatment group is compared with those before treatment, patient's FPG,2hPG are obviously lower, UAER is no significant changes. HbA1c reflects 8-12 weeks with the overall level of blood sugar, because the observation duration is less eight weeks, no comparing.2. Oxidative stress index and GAPDH results Compared with N group, the patients with diabetic microangiopathy MDA contents increased, GSH concentrations declined, SOD, GSH - PX, GAPDH activity declined, a significant statistically difference; Puerarin treatment group compared with those before treatment, patients with lower contents of MDA, GSH concentrations increased, SOD, GSH - PX, GAPDH activity increased, a statistically significant difference; Conventional treatment group compared with those before treatment MDA contents, GSH concentrations, SOD, GSH - PX, GAPDH activity without obviously changes; GAPDH activity has negatively correlated with MDA, and positively correlated with SOD,GSH,GSH - PX, also have statistical significance.Conclusion:1. A diabetic microangiopathy in patients with oxidative stress levels increased obviously..2. Puerarin can improve diabetic microangiopathy in patients with antioxidant capacity and inhibit oxidative stress, reduce urinary protein.3. GAPDH activity may indirectly reflect oxidative stress.