A Study Of Of Well-differentiated And Dedifferentiated Liposarcoma On Its Clinical Pathology And Moleccular Biology

Liposarcoma is the one of most common tumor of soft tissue of adult, take the 9.8%～16% of the sarcoma of soft tissue. each, position can occur the whole body, but see mainly after arms and legs and peritonaeum . It divides into on histology to well differentiated, dedifferentiated, myxoid, round cell, and pleomorphic five shape types liposarcoma, and according to its cell and member biology feature divide into it 3 big kinds: well-differentiated / dedifferentiated liposarcoma, myxoid and round cell liposarcoma and pleomorphic liposarcoma. Well differentiated in liposarcoma in contract take 40～45%, is easy to recur do not shift, malignant level is low can make progress is altitude malignant go to dedifferentiated liposarcoma. In dedifferentiated that takes liposarcoma approximately 10～15% can occur transfer and can lethal. Their feature on histology not is apparently, well differentiated liposarcoma with well fattiness tumor as well as dedifferentiated liposarcoma with malignant shuttle shape / much shape do not break up tumor distinguish is still a baffling problem. Now cell and member biology research well-differentiated / dedifferentiated liposarcoma the annular and(or) huge mark chromosome of type have the area of 12 q13-15 expand to increase, this area include some kinds of genes that cause tumor to occur such as MDM2, CDK4. In which most constant expand to increase son is MDM2 gene, it excludes can deliver tumor to occur besides can still take affect with P53 gene make tumor occur.We study observation to well-differentiated / dedifferentiated the organization pathologic feature of liposarcoma, immunity mark expresses characteristic, on detection member biology feature constant gene express, discuss their binary to melt well-differentiated / dedifferentiated the diagnosis of liposarcoma, the value of the judgement that distinguishes diagnosis and prognosis.Materials and methods:1. Materials: Test to collect 17 cases well-differentiated / dedifferentiated liposarcoma(in which 10 examples well-differentiated liposarcoma, 7 examples dedifferentiated liposarcoma), Additionally, collect to compare each 2 cases of 18 specimen examples, spindle cell liposarcoma, angiolipoma, lipoma, lipoblastomatosis, myxoid liposarcoma, pleomorphic liposarcoma, malignant fibrous histio-cytoma, rhabdomyosarcoma and fibrosarcoma.2. Methods: With immunity organize the method of melting detection CDK4 and MDM2 express, RT-PCR method detection MDM2 gene mRNA express.Reshlts:1. Clinical pathology characteristic In 10 WDLPSs male woman Zhi than is 7: 3, in position age 43.1 years old, occur in after peritonaeum and abdominal cavity 6 examples, Zong separate 1 cases, buttocks 1 case and the 2 cases of arms and legs. In 7 DDLPSs male woman Zhi than is 5: 2, in position age 58.1 years old ,occur in the arms and legs 3 cases, after peritonaeum 3 cases and shrewd rope 1 case. Follow to visit information: WDLPS 5 is regular to recur, 1 regular progresses to DDLPS, DDLPS 5 is regular to recur, 2 regular deathes.2. Immunity table type 17 cases well-differentiated / dedifferentiated liposarcoma and MDM2 express difference unusually is 82% and 76%. In comparing group, MDM2 expresses rate unusually to have discrepancy (P＜0.05). And CDK4 express rate do not have statistics meaning (P＞0.05).3. Member biology 10 cases well-differentiated / dedifferentiated liposarcoma draw RNA successfully, and detection go to the constant peculiar MDM2 gene that expresses, in the 9 cases of group, draw RNA only successfully, but do not detection go to MDM2 gene express. Conclusion:1. 17 cases well-differentiated / dedifferentiated liposarcoma MDM2 and CDK4 express difference unusually is 76% and 82%. Explanation they well-differentiated / dedifferentiated liposarcoma to occur them and the common member incident in development course, them with well-differentiated / dedifferentiated liposarcoma become sick mechanism is related closely.2. RT-PCR method detection well-differentiated / dedifferentiated liposarcoma paraffin bale bury the MDM2 gene in organization express is feasible, detection can distinguish the reference of diagnosis as clinical pathology diagnosis as a result.3. The method of immunohistochemistry to detect MDM2 protein can also assist well-differentiated / dedifferentiated liposarcoma clinical pathology diagnosis, action distinguish the reference of diagnosis. The protein of CDK4 express is not as good as MDM2 peculiar, as they distinguish the value of diagnosis is not large...