Mechanism Of Effect Of SF Injection Protect Myocardial Lesion Caused By Adriamycin Aardiotoxicity

Objective:Adriamycin(ADR) is general all purpose medication of tumor chems, which is broad anti-tumor zymogram and high remission rate. So it is generally used to treat multi-malignant tumor. However, ADR also bring about many by-effect of acu and chronicity in the therapeutic process. Among the total, its are the most severity to cause heart toxicity, which is limit to use ADR of large dose and long-term to degree. For this reason, it is worth approach and investigative topic that study and research drugs protecting cardiotoxicity, which is benefit to preferably play ADR anti-tumor pharmacodynamic action and to avoid its side effect.Shengfu inje0tion(SF) origin Ginseng and Aconite Praeparatae Decoction. Clinical discovered that SF could obviously lessen ADR induced heard toxicity when chems program certained ADR to make use of SF. Empirical study also indicated that SF could be against ADR caused lipid peroxidatio of myocardium. So its effect could protect cell envelope and myocardial preservation. To advance and expand mechanism of SF action of myocardial preservation, we study mechanism of SF protecting ADR induced cardiotoxicity from cell and molecular level. Therefore it will conduce abundant drugs protecting heard toxicity in the sphere of the china traditional medicine and play chinese patent medicine typical superiority.Methods:Acuting separation single myocardial cell, observed [Ca²⁺]_i of single myocardial cell measured by the Tillvision system, after myocardial cells were dyed with Fura-2/AM. Moreover, observed average of opening probability ratio and time of L- calcium channel in myocardial cells epimembranal. There were 5 parts of experimentation; Part one was to establish myocardial lesion model by adriamycin induced heart toxicity, to observe effect of myocardial lesion after the SF injection intervention; Part two was to observe [Ca²⁺]_i of single myocardial cells after SF injection intervention; Part three was to observe action endo-myocardial cell calcium releasing of caffeine; Part four was to observe effect of average of opening probability ratio and time of L- calcium channel in myocardial cells cpimembranal after SF injection intervention; Part five was to observe effect of average of opening probability ratio and time of L- calcium channel in myocardial cells epimembranal with the diff-concentration SF injection intervention.Results:1.Patho-results indicated that adriamycin induced heart toxicity cause myocardial arrangement disorder, intercellular substance generous hyperaemia and phlegmonosis cells infiltrating.At the same time there were cellular necrosis; Compared with control group(adriamycin induced heart toxicity), the group of SF injection intervention could lessen adriamycin induced heart toxicity. Patho-results indicated that myocardial arrangement order, intercellular substance not yet to see obviously hyperaemia and phlegrnonosis cells infiltrating.more over there were no cellular necrosis.2.With analysis and measured by the Tillvision system, it was observed that [Ca²⁺]_i of the group of ADR induced of single myocardial cell were heightened significantly compared with normal group(P<0.01); Compared with control group(the group of ADR induced), [Ca²⁺]_i of the group of SF injection intervention degraded significantly(P<0.01), even closely nomal level. But there were few obviously dose dependent.3.Compared with the group of SF injection no intervention, the group of SF injection intervention significantly inhibited endo-myocardial cell calcium releasing of caffeine induced(P<0.05).4.It was increased (P<0.01) that the group of ADR induced average of opening probability ratio and electric current of L-calcium channel in myocardial cells epimembranal, compared with that of nomal group.That of the group of SF injection intervention low dose and middle dose except high dose significantly (P<0.01)decreased compared with control group of ADR induced(P<0.01). And unknown significance compared with nomal group. But there were few obviously dose dependent.5.The results of the cliff-concentration SF injection effectiveness nomal myocardial cell hinted that SF high dose (35%-45%) could increase significantly (P<0.01)average of opening probability ratio of L- calcium channel in myocardial cells epimembranal,but it was unknown significance of average of opening electric current and time of L- calcium channel in myocardial cells epimembranal. Conclusion:One reason of mechanisms adriamycin induced heart toxicity are calcium overload of intracellular, which lead to a series of myocardial lesion; [Ca²⁺]_i of the group of SF injection intervention degraded significantly. There could be two aspects reasons. One could be mechanism of effect of SF injection inhibited endo-myocardial cell calcium releasing of sarcoplasmic reticuium. Another through protection and Stable cellular membrane, so reduced new calcium channel build, decrease average of opening probability ratio and electric current of L-calcium channel in myocardial cells epimembranal, and decease extra- calcium influx,That is also to say to decease Ca²⁺-induced Ca²⁺release. The results reduce intracellular [Ca²⁺]_i .There are still research wether or not affect intracellular calcium excretion and its conveying mechanisms. Through above experiment observation result,indicate that effect of SF injection could protect myocardial cell virulent caused by adriamycin.