The Influence Of Arsenic Trioxide And Polysaccharide To Tumor Cell Inhibitory Action And To Mouse Lymphocyte Multiplication Function

To investigate the effect of Arsenic Trioxide and Polysaccharide medicine to tie Rectal Cancer HCT-8cell and the person liver cancer BEL-7402cell growth inhibitory action, simultaneously discusses two medicines to the organismimmune cell (the spleen lymphocyte) the influence, from thesuppression tumor, enhances the organism immunity these two angles toexamine two medicines the functions, Provides the corresponding basis for the next step of two medicinesnew dosage-form preparation and two medicines in vivo experiment.The MTT was used to estimate the effect of Arsenic Trioxide and Polysaccharide to tie Rectal Cancer HCT-8cell and the person liver cancer BEL-7402cell growth inhibitory action. The MTT law examines the Arsenic Trioxide and Polysaccharide separately toin vitro raise mouse spleen lymphocyte increment function.Arsenic Trioxide in vitro has the inhibitory action to two tumors cells ,assumes the dependent relations with the density, After72h,HCT-8cell 7.5*10^-6these densities achieve half number of suppressions rate, BEL-7402cell 2.5*10^-6 these densities achieve half number of suppressions rate ; BEL-7402cell is more sensitive then HCT-8cell;In vitro Arsenic Trioxide also has the function which the suppression mouse lymphocytemultiplies, But compares with the Arsenic Trioxide to two kind of tumors cell sinhibitory action, it the inhibitory action which multiplies to the mouse lymphocyte is smaller than by far to the tumor cell inhibitory action. The Polysaccharide does not have the function in vitro to two tumors cells which the direct suppression tumor grows. But Polysaccharide in vitro has the function which the promotion mouse lymphocyte multiplies, the Polysaccharide may through the promotion spleen cell multiplication ,enhances the organism immunity, may reduces the Arsenic Trioxide to the organism injury, gets up reduces the poisonous function.