| Objective To determine whether direct transfer of the gene encoding antisense CD151 into artery could inhibit intima hyperplasia of vessel after balloon injury, in order to explore an effective target gene for gene therapy of arterial restenosis.Methods 60 SD adult male rats(350-400g), were randomly divided into simple injury group, gene therapeutic group, vector group and NS (normal saline) group, respectively.Experimental injury of vessel were built up in all animals by means of balloon catheter-induced injury in common carotid artery. In gene therapeutic group, the antisense CD151 cDNA was transfected into the segments of the injured carotid arteries either by perfusing pcDNA3.1-anti-CD151 plasmid plus liposomes in lumen, or by coating the surface of adventitia. As controls, pcDNA3.1(+) plasmid or NS also were delivered into injured segments by same procedure in vector group or NS group, respectively . The protein expression of CD151 in the arteries were evaluated by means of Immunohistochemical Assays. The effect of anti-sense CD151 cDNA on intimal hyperplasia were observed in pathological sections.Results Immunohistochemical assay in transfected tissues showed faint expression of CD151 in subintima in gene therapeutic group after 14 days, whereas in both control groups,there were intensive expression of CD151 protein. 28 days later, IT, IA, the ratio of IT/MT and IA/MA were all smaller significantly in gene therapeutic group than in both vector group and NS group respectively. Gene therapeutic group also showed more lumina areas of injured anteries in comparison with the two control groups significantly. Conclusion These results demonstrated that local direct transfer of gene encoding antisense CD 151 could reduce the intima hyperplasia, which also provided evidence that CD 151 plays an important role in mechanism of restenosis after balloon catheter- injured arteries.Postgraduate student : Wang Jiaping (Medicine,Cardiology)Directed by Professor Yang Jun...
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