| As one of the most common pathologies of primary glomerulonephritis which leads to children and adults'kidney diseases to last until terminal stage of the renal failure, Focal segmental glomerulosclerosis(FSGS) shows diversity on clinical manifestation. Glomerular sclerosis goes from bad to worse with the disease progress, depauperation of renal tubule and diffuse disposition of interstitial fibrosis. Study shows that pathological changes of renal interstitium have the same contribution to renal function changes and kidney disease prognosis, how to block up or postpone the progress of FSGS specially how to interfere in renal fibrosis process has become a research hot spot recently. Two kinds of materials that affect the renal fibrosis process have been found in renal tissues depending on molecular biology technology, one is positive regulation factors, the other is negative regulation factors. There is some unvarying association between TGF-βand HGF in renal fibrosis process as clinic and animal test shows.Immunity class test and analysis has been carried out to study the representation and mutual relationship between TGF-βand HGF in children's renal tissues.Aim:1. To study the representation of TGF-βand HGF in renal tissues of thin basement membrane nephropathy2. To study the representation of TGF-βand HGF in renal tissues of FSGS3. To explore the interrelationship between representation differences of TGF-βand HGF in renal tissues through FSGS evolution.4. To explore the interrelationship between Alb and NAG in children'urineMethod:1. experimental group enactment: 35 cases diagnosed primary nephrotic syndrome together with FSGS were choose.(22 cases-no pathological changes to renal interstitium, 13 cases- light renal interstitial fibrosis)2. Control group enactment: 26 cases diagnosed isolated hematuresis together with thin basement membrane nephropathy3. Cell Factors representation in renal tissues: immunoenzymic–SP method was applied.4. Semiquantitative analysis to TGF and HGF in nephridial tissue: film reading was viewed by Olympus microscope and Medical image analysis software was Used to determine the masculine area of HGF or TGF-βand image intensity; then the Immunity class index was defined as masculine area×image intensity.5. Statistical treatment Software used: SPSS 11.5 Data Representation: x±s Inter-Class Comparison: t test (P<0.05) Intra-Class Comparison: Pearson test (P<0.05 OR P<0.01)Result:1.Interrelationship between HGF and TGF-βin nephridial tissues of Control group(1) Representation to HGF (26 Cases): Immunity class index of Glomerular mesangium: 2.34±0.19, Immunity class index of nephric tubule: 3.29±0.47.(2)Representation to HGF (26 Cases): Immunity class index of Glomerular mesangium: 3.52±0.55, Immunity class index of nephric tubule: 2.81±0.65.2.Interrelationship between HGF and TGF-βin nephridial tissues of Test group2.1 Test group 1(there is no affection to renal interstitium)(1) Representation to HGF (22 Cases): Immunity class index of Glomerular mesangium: 15.31±0.95, Immunity class index of nephric tubule: 16.40±1.44.(2)Representation to HGF (22 Cases): Immunity class index of Glomerular mesangium: 16.78±0.83,, Immunity class index of nephric tubule: 18.66±1.50.2.2 Test group 2(there is light affection to renal interstitium)(1) Representation to HGF (13 Cases): Immunity class index of Glomerular mesangium: 14.91±1.37, Immunity class index of nephric tubule: 15.62±1.03.(2)Representation to HGF (13 Cases): Immunity class index of Glomerular mesangium: 19.38±1.39, Immunity class index of nephric tubule: 21.07±1.43. Conclusion:1. HGF and TGF-βin renal glomeruli and renal interstitium exist in both test groups and control group.2. comparing to control group, representation of HGF and TGF-βincrease obviously(P<0.05) with pathology alteration both in test group 1 and 2.3. comparing to test group 1,HGF in renal tissues decreases obviously((P>0.05) and TGF-βincreases manifestly(P<0.05) with pathology alteration.4. HGF and TGF-βin renal glomeruli and renal interstitium presents negative correlation(r<0), especially in renal interstitium(P<0.01).
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