The Expression And Clinical Significance Of PPARγ And MMP-2,MMP-9 In Gastric Carcer

ObjectiveTo study the expression of MMP-2 (matrix metalloproteinase 2),MMP-9 (matrix metalloproteinase 9) and PPARγ(peroxisomeproliferators activated receptorγ) in gastric adenocarcinoma and search predicting markers for lymph node metastasis in gastric adenocarcinoma. To investigate the relationship among MMP-2,MMP-9 and PPARγin the development of gastric carcinoma.MethodsTumor specimens were collected from 75 gastric adenocarcinoma cases and 20 cases non-tumor gastric tissue in which lymph nodes were also checked.All tumor specimens were consecutively cut into serial sections and examined by microscope. Total number of metastatic lymph nodes was counted. And staging was conducted according to the Classification of UICC/AJCC standard. N0:no lymph node metastasis, N1:1-6 lymph nodesmetastasis, N2:7-15 lymph nodes metastasis, N3:＞15 lymph nodes metastasis. The expression of MMP-2,MMP-9 and PPARγwas detected on the section of tissue microarray by immunohistochemistry.All data were processed using SAS 8.1 and SPSS 13.0 software. The difference of the expression of MMP-2,MMP-9 and PPARγin gastric adenocarcinoma with or without lymph node metastasis was processed usingχ~2-test, and P＜0.05 was considered statistical significance.Results 1,After using immunohistochemistry stain, MMP-2,MMP-9 and PPARγ, there were 75 cases left. The usable rate was 100％.2,The positive MMP-2,MMP-9 and PPARγexpression rate has positive correlation with infiltrated depth,lymphatic metastasis,clinical stage of gastric cancer (P＜0.05), and with deepening of the tumorous infiltrated depth, generating lymphatic metastasis, happening of far distance metastasis and increasing of clinical stage, positive MMP-2,MMP-9 and PPARγexpression rate rises obviously. Positive MMP-2,MMP-9 and PPARγexpression rate is negatively related with pathological differentiated degree of tumor (P＜0.05);it rises when pathological differentiated degree of tumor decreases.3,The expression of PPARγwas negative related to that of MMP-2, MMP-9 protein (r=-0.336,-0.401, P＜0.05).Conclusions1,Expression of PPARγand MMP-2,MMP-9 is abnormal in the gastric cancerous tissue.2,The close relationship between positive expression rate of PPARγ,MMP-2,MMP-9 in the gastric cancerous tissue and tumorous lymphatic metastasis,clinicalstage,pathological differentiated degree shows that they have suppressed or promotive effect in the development and infiltrating metastasis of gastric carcinoma.3,Negative correlation between PPARγand MMP-2,MMP-9 expression displays that they restrict each other in the process of gastric caicinoma happening,growth,infliltration and metastasis.4,PPARγand MMP-2,MMP-9 could be three markers to the diagnose and predict the prognosis of gastric cancer.