| Introduction and ObjectiveThe cholangiocarcinoma is a common malignant tumor. The symptoms appears late, the early diagnosis is difficult, and the rate of surgically radical excision is low, so the prognosis is bad. Therefore, investigation of the mechanism and finding the effective therapeutic method are important to elevate the diagnosis and treatment of the cholangiocarcinoma. The Toll-like receptors(TLRs) are newly discovered natural immunologic receptors. Among of them, was firstly found TLR4. The most basic function of TLRs as type 1 transmembrane protein is tranporting the signal transmenbranely. On one hand,the binding of TLRs and corresponding ligands(such as LPS, HSP) could activate immunologic cells and facilitate them to secrete amount of inflammative factors and chemotatic factors, which educe the anti-pathologic microoganism and the anti-tumor ability. On the other hand, TLRs were expressed on some tumor cells which would secrete amount of cytokines while stimulated by special ligands to improve the micro-environment for tumor survival and help tumor cells escape immune surveil. There were important theory significance and amplication value to further investigate TLRs of tumor cells for revealing the mechanism of tumors and practicing immunal treatment. Heat shock protein( HSP) is a highly conservative protein generally exist in living nature. Its main biological function is to protect the proteins that are necessary in cellular movement, and to sustain the life of cells in the stringent state. HSP70 is the most important member in the heat shock protein with a molecular weight of 70KD. It is highly expressed in most tumor cells, regulates the cell proliferation, conteracts cell apoptosis. In current study, immunologic histochemistry methods was used to investigate the expression of TLR4 and HSP70 in cholangiiocarcinoma, reveal the relationship of the two proteins in cholangiocarcinoma and whether existing correlation between of them.MethodsThe 50 specimens of cholangiocarcinoma were selected from January, 1999 to December, 2005 at the First Hospital of China Medical University, and all pathologically validated. Para-tumor tissue of biliary tract was from 31 cases of radical excision., and no tumor cells were found pathologically. We detected the expression of TLR4 and HSP70 in the tumor and para-tumor tissue in immunologic histochemistry methods, and utilized the image analysis software (Image-pro Plus 5.0) to count and analysis the specimens. Statistic analysis was completed with statistic software SPSS11.0, and K-S test, analysis of variance, SNK test and t test were used.The analysis of correlation of each factor used linear correlation. P<0.05, was as the level for statistical significance.ResultsTLR4 was mainly expressed on cell membrane and HSP70 mainly on cytoplasm. The expression of TLR4 and HSP70 was significantly higher in cholangiocarcinoma tissue than in para-tumor tissue (P<0.05). Further study indicated the expression of TLR4 had no relationship with pathological differentiation degree, sex, TNM classification (P>0.05) .As for HSP70, the expression of HSP70 has close relationship with the initiation and development of cholangiocarcinoma, yet had no relationship with age, sex, and TNM classification (P>0.05) .The expression of HSP70 and TLR4 has no significant correlation.ConclusionThe expression of TLR4 in the cholangiocarcinoma tissue was significantly higher than that in the para-tumor tissue, yet had no relation with age, sex, pathological differantiation degree and TNM classification. The expression of HSP70 in the tumor tissue was higher than that in the para-tumor tissue. But the expression of HSP70 were highly correlated with the pathologic differetiation degree of tumer, the lower differetiation degree, the more expression, but had no relationship with the age, sex, and TNM classification, The correlation analysis of the expression of TLR4 and HSP70 revealed that there was no relationship between them.
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