Experimental Study Of Repairing Of The Peripheral Erve Defect With Acellular Heterogeneous Nerve Transplant

ObjectiveShort distant defected peripheral nerve can regenerate by repairing and suturing, but the repairing of long distant nerve defect is still a topic.With the development of tissue engineering many scholars explored various kinds of transplants to repair the nerve defect.Among them, homogeneous acellular transplant gained well development, and it took off cells and remained the ecto-base and kept fine analogue.If the transplant was used to repair the nerve defect, its histocompatibility kept well and the sensory function and the motor function could recover. However,the source of donator will be restricted in clinical application. In order to solve the issue,heterogeneous nervous acellular transplant was used in the experiment to repair the defect,and we detected the immunological rejection in the donator at the same time.We hoped to offer experimental data for clinical nerve repairing .Materials and Methods1. Experimental animals20 female Wistar rats weighed 200～220g,and 5 rabbits weighed 4.5Kg. The animals were supplied by China Medical University.2. Preparing of acellular transplantWe took off the cell from the peripheral nerve of the rabbits by chemical method,and used them as transplant to repair the sciatic nerve 1 cm defect of rats in the condition of asepsis. 3. Observed index and detected methods(1) Observing the gross morphology of lower limb of operated side of the rats and the recovery of the sensory and the motor function.(2) Observing the change of lymphocyte of the rats by LiuShi staining,then comparing with the normal rats.(3) Observing the recover of the myoceptor of the gastrocnemius of the operated side by histochemistry staining three months after the operation.(4) Observing the recover of nerve fiber in the transplant by toluidine blue staining.(5) Observing the recover of nerve fiber by transmission electron microscope.Results1. Three months after the operation, the toes of the rat on the operated side could be found to separate and walk powerfully,and their footplate could escape when acupunctured.2. There was no obvious change of lymphocyte of the rats after the operation.3. Three months after the operation, the regenerated nerve fibers and myoceptor in the fibers of gastrocnemius could be observed.4. A great deal of regenerated nerve fibers in the transplant could be observed.Conclusions1. Heterogeneous nerve transplant can repair nerve defect after they were taken off cells, and the immunologic reject reaction did not happen in the receptor.2. There was no obvious change of lymphocyte of the rats after the operation of heterogeneous nerve transplant,3. It can re-establish the construction of nerve-muscle and recover the functions.