The Effects Of WISP3 On Proliferation And Differentiation In Chondrocytes

Object: To observe the effects of recombinant human WISP3 (RhWIS3) on the proliferation and differentiation in human immortalized chondrocytes cell lines T/C-28a2. To investigate whether the effects is through the IGF signaling pathway.Methods: T/C-28a2 cell lines were treated with various RhWIS3 concentrations with or without recombinant human IGF-1 (RhIGF-1),and the proliferation of cell lines was determined by [~3H]-Tdr incorpotation. The expression of collagen II of chondrocytes treated by RhWISP3 was determined by Western blot. The cell lines were treated with an IGF-1 signaling pathway inhibitor JBl,and the expression of collagen II of chondrocytes was determined by Western blot.Results: RhWIS3 had no effect on the proliferation of T/C-28a2 cell lines. RhIGF-1 promoted proliferation of cell lines significantly, and RhWIS3 also had no effect on the proliferation of cell lines treated with RhIGF-1. RhWIS3 increases the production of collagen II in a dose dependent manner. RhIGF-1 also had effect on production of collagen II JB1 inhibited the increasing expression of collagen II treated with RhIGF-l,but has no effect on increasing expression of collagen II. treated with RhWIS3.Conclusion: RhWIS3 has no effect on the proliferation of T/C-28a2 cell lines,but increases the production of collagen II in a dose dependent manner,possibaly through IGF-independent signaling.