Imaging Of Pancreas In Mice By Ultrasmall Superparamagnetic Iron Oxide (USPIO)-enhanced MRI

Objective: To establish a ultrasmall superparamagnetic iron oxide (USPIO)-enhanced magnetic resonance imaging (MRI) technique for imaging and quantifying of pancreas in mice.Methods: The transverse, sagital and coronal image of pancreas in mice under different means of anesthesia and fasten was determined by spin echo sequence using 1.5-tesla (T) MRI. And the image of pancreases in mice was analyzed by injecting dimeglumine gadopentetic acid into the mice pancreas. Finally, the T2-value changes of mice pancreas were evaluated by paired echo sequence using USPIO-enhanced MRI.Results: The transverse, sagital and coronal image of abdomen in mice was achieved dearly by spin echo sequence, and most of coeliac organs of mice can be indentified by using 1.5-T MRI. The pancreas in mice was accurately located in transverse image. And the T2-value of mice pancreas desceased after injection of USPIO.Conclusions: USPIO-enhanced MRI technique for imaging and quantifying of pancreas in mice by using clinically available 1.5-T scanner was successfully established, being a foundation for further development of noninvasive imaging insulitis in type 1 diabetes.