Role Of Hepatocyte Growth Factor And Transforming Growth Factor In Diabetic Cardiomyopathy

Objective:The changes of the concentrations of serum HGF and TGF were measured by ELISA in the subjects of different phases of type 1 diabetes mellitus and normal control.The levels of fast blood glucose(FBG),blood triglyceride(Tri),blood total cholesterol(Cho),fast blood insulin(FINS),C-peptide et al were also examined.Comparing with these parameters between the two groups and among the different phases we can conclude the changes of HGF,TGFβ₁ and diabetic cardiomyopathy.Furthermore,the glomerular changes in different phases were observed morphologically to document the degrees of diabebic cardiomyopathy.The changes of serum levels of HGF and TGFβ₁under different degrees of diabebic cardiomyopathy.determined role of the two factors in the progression of.diabebic cardiomyopathy.The experiment may be valuable to provide a new method to diagnose early and therapy effectively diabebic cardiomyopathy.Methods:48 Wistar rats were divided randomly into two groups.There were 24 in normal control group(NC)and 24 in diabetes mellitus group(DM).The subjects in DM group were injected intraperitoneally with dose streptozotocin(STZ,55mg/kg)while the ones in NC group were injected intraperitoneally with the similar dose of citrate buffer,six weeks,ten weeks and forteen weeks administration,there were eight rats in both groups respectively killed.The blood samples were obtained to measure the levels of HGF,TGFβ₁,Cho,Tri,et al.the heart samples were used to analyse morphologically.We compare these data between the two groups and among the different stages with t-test,use analysis of variance to compare these date among the difference stages in DM and use Linear correlation to reveal the role of HGF and TGFβ₁ in the pathogenesy of diabebic cardiomyopathy.Results:1.There was a difference in the level of fast blood glucose between NC and DM group,The difference was significant after injected intraperitoneally with STZ and the level of FBG was higher than 16.8 mmol/L in DM group.In addition,there were a significant difference in the levels of triglyceride and cholesterol between the two groups2.Compared with NC,there were significant differences in the level of FINS and C -peptide(p＜0.01).The differences between the two groups had existed for the whole course of the experiment.The phenomena are also similar to type 1 diabetes mellitus.3 In DM group,the changes of myocardium ultrastructure were obvious,myocardium mitochondria was edematous,and myocardium endothelial ground membrane was thincker at month? After injected STZ(p＜0.01),myocardium cell was bigger than NC.4 After admimistrated with STZ six weeks later,the TGFβ₁ was higher than NC(p＜0.05), ten weeks later,the TGFβ₁ was higher than it of six weeks(p＜0.01),forteen weeks later,the TGFβ₁ was higher than it often weeks.5,After admimistrated with STZ six weeks later,the TGFβ₁ was higher than NC (p＜0.05),then it decreased gradually.Conclusion:1.The rats were given dose STZ to destroy beta cells.This model closely mimics the features of human type 1 diabetes mellitus.2.the level of TGFβ₁ of DM group was much higher than normal control.The levels of serum TGFβ₁ increased continually during the whole experiment.According these we can conclude that TGFβ₁ may reveal promotional role in progression of diabebic cardiomyopathy3.the level of HGF of DM group was much higher than normal control.Furthermore,the levels of serum HGF increased fastly at the beginning of DM and then it decreased gradually.According these,we can conclude that HGF may play a protective role in the diabebic cardiomyopathy.4.There was a significant negative correlation between HGF and TGFβ₁ of DM group.They play different roles in the progression ofdiabebic cardiomyopathy.