| Objective: Overtraining can induce the body's injury. The functions of many organs including kidney have been affected. To investigate the mechanism of overtraining-induced acute kidney injury(OTIAKI) model was established by exhaustive swimming.The renal tissue pathological changes were observed by light microscopy. Immunohistochemistry and ELISA were performed to evaluate the expression of hypoxia inducible factor and erythropoietin. In addition, the protective effect of anisodamine was also observed in the present study.Methods: 1. 36 male Wistar rats were randomly divided into 6 groups: CN (quiet control group), ESI (immediately after exhaustive swimming group), ES6h (6hours after exhaustive swimming group), ES24h (24hours after exhaustive swimming group), ADgroup(the rat of AD received a single intraporitonenl injection of anisodamine at a dose of 10mg/kg at 10 minute before swimming and then swimed to exhausted condition, the rats of AD were sacrificed at 6 hour (AD6h) and at 24 hour (AD24h) after exhaustive swimimg ) 2. A animal model of OTIAKI was induced by exhaustive swimming : Rats of each groups were given adapt swimming 30 minutes at one day before the experimental day, then were put into the water till exhausted. 3. Sample: 6 rats of each group were sacrificed after exhausted at different times . Kidneys of the rats were removed. 4.The expression of hypoxia inducible factor and erythropoietin were detected by Immunohistochemical staining and ELISA. All data were expressed as x±s, T test and F test was used to analyzed the difference between the two groups and among groups. All data was finished with SPSS 12.0 software.Results: 1. Compared with that of control rats , the ischemic changes was observed in the renal tissure of the rats that accepted overtraining by light microscopy, the morphologic changes mainly occurred at 24 hour. No morphologic changes can been observed in AD rats. 2. There was only little expression of hypoxia inducible factor in the normal kidneys of rats, but the expression was increased after exhaustive swimming, especially at 6 hours , and decreased at 24 hours. 3. The erythropoietin was slightly expressed in normal kidneys, and the expression was increased after exhaustive swimming, especially at 6 hours, and also decreased at 24 hours. After use of anisodamine, the expression of HIF-1αand EPO was increased significantly compared with that of control rats. 4. The expression of EPO in the serum of rats was increased after exhaustive swimming, especially at 6 hours, and decreased at 24 hours. After use of anisodamine, the expression of EPO was also increased compared with ES rats.Conclusions: 1. In ESI and ES6h rats the expression of HIF-1αand EPO was increased remarkably in renal tissue, and the expression of EPO in the serum was also increased, which could be the protective mechamism on kidney injury induced by exhaustive swiming. The expression of HIF-1αand EPO was decreased at 24 hours compared with 6 hours, the expression of EPO in the serum of rats was also decreased, which indicated that the protective effects were decreased. 2. The rats which used anisodamine the expression of HIF-1αand EPO was increased, and the expression of EPO in the serum of rats was also increased, which indicated that anisodamine have obvious protective effects on OTIAKI by regulating the expression of HIF-1αand EPO in kidney . |
PDF Full Text Request