| Objective: To investigate the changes of the distribution, functional status andinfiltrated density of DC in prostate cancer microenviroment, and analyze theprobalble reasons of the above changes. Then discuss the relationship betweeninfiltrated density of DC and the serum PSA,Gleason score,the LN metastatis andthe prognosis of the patients. To offer evidences in theory to the immunotherapy ofthe prostate cancer.Method: The lineage of 20 benign prostate hyperplasia (BPH), 20 prostaticintraepithelial neoplasm (PIN), 40 prostate cancer (Pca) and pelvis LN were studied.With IHC SP method, the expressions of S100+DC, CD83+DC and CD45RO+Tlymphocyte was displayed. Then the distribution of the above three cells werereviewed, the infiltrated density of them calculated, and the relationship among themanalyzed. The relationships between the infiltrated density of the three cells in thepatients with cancer and their serum PSA,Gleason score,the LN metastatis and theprognosis were also analyzed. Finally, the expressions of VEGF and TGF-βweredetected in the tissues of prostate cancer, and their relationships with DC wereanalyzed.Results:1. In different degree, the expressions of S100+DC, CD83+DC and CD45RO+Tlymphocyte can be detected in the three tissues, however, there is no statisticaldifference among their positive-expression rate (x~2=0.989, 1.146, 0.949 P＞0.05).2. There is statistical difference among the infiltrated densities of S100+DC,CDg3+DC and CD45RO+T lymphocyte in the three prostate tissues (P＜0.05).Respectively, in BPH and PIN, BPH and Pca, PIN and Pca, there was significantlystatistical difference (P=0.00＜0.01) for the infiltrated density of S100+DC; there wasstatistical difference in BPH and Pca (P=0.016＜0.05) for the density of CD83+DC,PIN and Pca (P=0.034＜0.05), however, there is no statistical difference in BPH and PIN (P=1.00＞0.05). In the three tissues, there was negative relationship between thedensities of S100+DC and CD83+DC with the density of CD45RO+T lymphocyte(BPH: r_s=0.938, 0919 P＜0.05; PIN: r_s=0.912, 0.885 P＜0.05; Pca:r_s=0.960, 0.831P＜0.05).3. In the prostate cancer tissue, S100+DC are mainly distributed in theintraepithelium and the margin of the advancing cancer (t=9.309, P＜0.01). CD83+DCare mainly distributed in the tumor stroma (t=8.748, P＜0.01). CD45RO+Tlymphocytes are also mainly distributed in the tumor stroma (t=7.627, P＜0.01).4. For prostate cancer, there is a negative relationship between the infiltrateddensity of S100+DC, CD83+DC and the Gteason score, serum PSA (r_s=-0.806, -0.781P＜0.01). There was significantly statistical difference for the infiltrated density ofS100+DC, CD83+DC, whether there was lymph node metastasis (P＜0.05).5. For patients with prostate cancer, there is no statistical difference in survivaltime between the S100+one and the S100-one. However, there is statistical differencebetween the CD83+one and the CD83-one.6. For S100+DC, there is no statistical difference between the VEGF+ and VEGF-cancer specimen (P＞0.05), while for CD83+DC, there is statistical difference andTGF-β+ and TGF-β-specimens (P＜0.05) in patients with prostate cancer.Conclusion:1. In the prostate cancer microenvironment, the infiltrated density and activity ofDC decreased, it offers reasonable theory to the immunity therapy based on DC inprostate cancer.2. In the prostate cancer tissue, S100+DC are mainly distributed in the cancerepithelium and the margin of the advancing cancer, CD83+DC and CD45RO+Tmainly in the tumor stroma.3. The infiltrated density and activity of DC decrease with the increase of serumPSA, Gleason score in prostate cancer. The activated DC may determine theprognosis of the patients with prostate cancer.4. VEGF and TGF-βmay inhibit the mature of DC in prostate cancer.
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