Toxic Effect Of Sodium Nitrite On Sertoli Cell Of Rat

Objective To investigate the toxic effect of sodium nitrite on expression of vimentin and DNA injury in primary cultured sertoli cell and provide reasonable suggestions to prevent male sterility.Methods The model of primary cultured sertoli cell was set up. The toxicity of sodium nitrite on primary cultured sertoli cell was examed through MTT and IC₅₀ was calculated through KO's as the highest dosage. Then the dosage was diluted to three dosages. In this study the effect of sodium nitrite on DNA and vimentin of sertoli cell was examed by SCGE and immunocytochemistry.Results1. When the dosage of sodium nitrite was 0.015mg/ml, the cell would be damaged and there was dose-effect relationship between the dosages of sodium nitrite and inhibition ratio of sertoli cell. IC₅₀was calculated by Ko's method. IC₅₀ was 1.17mg/ml and standard deviation was 1.14mg/ml.2. The comet tail of 0.0015mg/ml group was not obvious, but the comet tail of 0.015mg/ml,0.15mg/ml and 1.5mg/ml groups were very obvious. The rate of comet tail and tail length of experiment groups were higher than those of negative group.3. The effect of sodium nitrite on vimentin was examed through immunocytochemistry. The average gray scales of experiment groups were higher than those of negative group. There was tendency between the dosages and the average gray scale.Conclusions Sodium nitrite could cause DNA injury of sertoli cell and there is tendency between dosage and effect. Sodium nitrite could affect vimentin's expression of sertoli cell.