Experimental Study Of C-erbB-2 Anti-sense Probe On Ovarian Carcinoma Cell Line SKOV3

Objective: To investigate the inhibition effect of C-erbB-2 senseoligodeoxynucleotides(SODN),antisense oligodeoxynucleotides (ASODN),antisensepeptide nucleic acid(ASPNA) and radionuclide antisense therapy, to human ovariancarcinoma cell.Methods: According to different drugs, the experiment groups are as follows: the controlgroups (the normal control,the liposome control,DDP control) oligonucleotides groups(SODN,ASODN,liposome-mediated SODN,liposome-mediated ASODN), PNAgroups(ASPNA,liposome-mediated ASPNA). ¹²⁵I-labeled groups (¹²⁵I-Na,¹²⁵I-ASPNA).The cell proliferation was observed by MTT assay and cloning formation test afterliposome-mediated transfection with 3d,5d and 7d respectively.RESULTS: When transfected compound concentration at 0.5nmol/ml: the OD-value ofthe control group were 0.363±0.046,0.661±0.095å’Œ0.989±0.143 at 3d,5d,7drespectively. The number of colony formation was 40.3±6.1. The OD-value ofliposome-mediated ASODN were 0.191±0.023,0.212±0.029,0.420±0.034 at 3d,5d,7d respectively. The number of colony formation was 2.3±1.3. With the control group,difference between the two groups was statistically significant (P＜0.05). The OD-value ofDDP group were 0.223±0.036,0.273±0.031,0.486±0.052 at 3d,5d,7d respectively.The number of colony formation was 3.6±2.1. With liposome-mediated ASODN, thedifference was not significant (P＞0.05). According to liposome-mediated antisensepeptide nucleic acid group, the OD-value were 0.336±0.048,0.589±0.062,0.886±0.086at3d,5d,7d respectively. The number of colony formation were 33.4±3.5. With thecontrol group, the difference was not statistically significant (P＞0.05). According to¹²⁵I-ASPNA, the OD-value were 0.336±0.048,0.487±0.034,0.802±0.099 at 3d,5d, 7d respectively. The number of colony formation was 34.6±4.1. With the controls, thedifference was not statistically significant (P＞0.05).CONCLUSIONS: 1. Lipid-mediated c-erbB-2 antisense oligonucleotide show inhibition tothe growth and proliferation of the SKOV3 cell. 2. Lipid-mediated c-erbB-2 Antisenseoligonucleotide inhibits cell proliferation over the unused liposomal transfection, whichsuggest liposomes can effectively promote antisense oligonucleotide cell transduction. 3.Lipid-mediated c-erbB-2 antisense oligonucleotide shows no statistically significant withDDP group from OD value of 3d,5d,7d, suggesting good prospects of antisenseoligonucleotides in the treatment of ovarian cancer.