| Objective: Human interferon lamdal (hIFN-λ1) and epsilon (hIFN-ε) have recently beenidentified as new types of interferons. Study for both interferons has been limited so far.Thus, we have obtained full-length cDNAs for both interferons by RT-PCR amplification;we cloned the cDNAs into eukaryotic expression vector and transfect the resultingconstructs into mammalian cells. Furthermore, biological activity of proteins expressedin the vector was investigated in mammalian cells.Methods: Total RNA was isolated from mammalian cells stimulated by cytokines.Full-length human hIFN-λ1 or hIFN-εcDNA was obtained by RT-PCR and cloned intothe vector pcDNA3.1/myc-his(-)A. First we constructed the fusion expression vectorpcDNA3.1A-hIFN-λ1-Hisand pcDNA3.1A-hIFN-ε1-His by PCR, then transfected theseplasmids in to WI-38 cells (human embryonic lung cell) with liposome. After screeningthese cells with G418, we have identified the expression system with PCR, RT-PCR anddot-ELISA. And we have studied and compared the antivirus activities of rhIFN-λ1-Hisand rhIFN-ε1-His to HSV-1, PolioV and AD3, meanwhile MTT assay was used to detecttheir bioactivities towards tumor cells: SHG-44, MDA-MB-231 and SMMC-7721. RT-PCR relative quantitative method use to examine expression of antivirus protein MxA inthe WI-38 and SHG-44 cells.Results: The transfected WI-38 cells can stably express the proteins of rhIFN-λ1-His andrhIFN-ε1-His, both of which suppressed virus of HSV-â… ,PolioV,Ad3 and the tumorcells' growth of SHG-44, MDA-MB-231 and SMMC-7721, And both of which caninduce WI-38 and SHG-44 to produce protein MxA; However, the antivirus activity,antiproliferative activity of rhIFN-λ1-His and the bioactivities of protein Max induced byrhIFN-λ1-His are all more powerful than that of rhIFN-ε1-His. Conclusion: The antiviral molecular mechanisms of both hIFN-λ1 and hIFN-ε1 arerelative to MxA. This research established foundation for further study on thebioactivities and mechanism of action of hIFN-λ1 and hIFN-ε1.
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