Pharmacodynamics Study Of Jingangteng Dispersible Tablet On Experimental Chronic Pelvic Inflammatory Disease

BackgroundChronic Pelvic Inflammatory Disease (CPID) is a kind of chronic inflammatorydisease takes place at female endogynium, peripheral connective tissue and pelvicperitoneum. Including endometritis,salpingitis,tubo-ovarian abscess and peritonitis,etc. CPID is a common and frequently occurring disease in the department ofgynecology.Jingangteng, which is also called Rhizoma smilacinus (RS), is the root ofSmilax china L..It has the pharmacologic actions such as anti-inflammatory,analgesic and activating blood circulation to dissipate blood stasis. There are manykinds of dosage forms such as syrup, particle, capsule and tablet in clinical to curegynaecopathia inflammatory disease and tubercle. It has been testified thatJingangteng has the precisely therapeutical effect on CPID by a long time clinicalverification, and there is no Jingangteng Dispersible Tablet in the market.Jingangteng Dispersible Tablet (JDT), which was originated from Jingangtengcapsule of 'Chinese Pharmacopeia' 2005, is a kind of solid dosage form with quickerdisaggregation,faster dissolve and higher bioavailability than the capsule and tablet. PurposeTo prove the Pharmacodynamics of JDT in experimental CPID and providescientific evidence for its clinical application.Methods1 Observed the effect of JDT on CPID model rat induced by phenol glue.1.1 Injected phenol glue into the right side of uterui in rats to make CPIDmodels, seven days later, the operated rats were cured by continuous fourteen days'lavage.1.2 After the lavage, obeserved the effect of JDT on the blood reheology inCPID model rats by drawing the blood from them.1.3 After the blood rheology was measured, observed the changes of the rats'uteri by naked eyes, took photos, and studied the effect of JDT on the uteri' atatomyin CPID model rats by describing the pathological changes.1.4 Picked out the rats' uteri to make pathological section, observed inmicroscope and took photos, and then studied the effect of JDT on the endometriahistomorphology in CPID model rats by classified the pathological changes.2 Observed the antiinflammatory and analgesic effect of JDT.2.1 After continuous lavaging for seven days, made the swelling of pinnamodels induced by xylene in mice, and observed the effect of JDT on the models.2.2 After continuous lavaging for five days, made the swelling of the voixpedis models induced by albumen in rats, and observed the effect of JDT on themodels.2.3 Made the cotton ball granuloma models in rats, after continuous lavagingfor seven days, observed the effect of JDT on the models.2.4 After continuous lavaging for seven days, the writhing experiment inducedby acetic acid in mice was done to observe the effect of JDT.3 The acute toxicity experiment of JDT in miceSingle lavaged the mice with the maximum dosage 936.0 g/kg according to thecrude drug, that was the utmost soluble 23.4 g/ml and was as 80 times dosage as theclinical treatment, and the maximum lavage volume was 0.8 ml/20g (weight). Observed the acute toxicity reaction for seven days.4 Statistical TreatmentsThe data was dealed with by SPSS10.0 statistics system. The measurement datawas expressed as (?)±s. Paired-samples T test was used to compare the sumclassification data of the left and the rigth uteri histomorphology in the operated rats,Repeat measured data ANOVA and One-way ANOVA were used to deal with thedata of the swelling of the voix pedis of the rat. The multiple comparisons of otherdata was dealed with by One-way ANOVA. LSD and T₂ test were took to comparethe two groups when the data meeted homoscedasticity or not. Two-sided test wasused to indicate significance test by P figure, P＜0.05 meant significant difference.Results1 JDT had an utility therapeutical effect on CPID model rat induced byphenol glue.1.1 Phenol glue could be used to induce the CPID model.1.2 JDT could improve the indexes of the whole blood consistency of theblood rheology of CPID model rats significantly, the P fingure are 0.000. JDT wasmore effective than Jingangteng Capsule (JC) as a positive control in treatment. Thetherapeutical effect of JDT became better as the dosage increasing.1.3 JDT could significantly improve the atatomy pathological changes of theuteri in CPID model rats, and relieved the swelling and congestion of theinflammatory uteri in rats. JDT was more effective than JC as a positive control intreatment.1.4 JDT could significantly improved the endometria histomorphology of theuteri in CPID model rats,the sum data (F=28.107, P=0.000). JDT was more effectivethan JC as a positive control in treatment. The therapeutical effect of JDT becamebetter as the dosage rose.2 JDT had a good antiinflammatory and analgescic effect.2.1 JDT Could significantly inhibit the swelling of pinna(F=3.039, P=0.017),which indicated that JDT had a good anti-acute inflammation effect. JDT was moreeffective than JC as a positive cintrol. 2.2 JDT could significantly inhibit the swelling of voix pedis(F=4.979,P=0.001), which indicated that JDT had a good anti-acute inflammation effect. JDTwas more effective than JC as a positive control. The inhibition effect became betteras the dosage increasing.2.3 JDT could significantly inhibit the granulomatous accrementition inducedby cotton ball(F=2.668, P=0.032),which indicated that JDT had a good anti-chronicinflammatory effect. JDT was more effective than JC as a positive control. Theinhibition effect became better as the dosage increasing.2.4 JDT could significantly inhibit the writhing response of the mice(F=3.151,P=0.014), which indicated that JDT had a strong analgesic effect. JDT was equal toJC as a positive control.3 The mice didn't show the acute toxicity response, the maximum tolerateddosage according to the crude drug was 936.0g/kg, which was as 80 times as theclinical treatment.ConclusionThe experiment above-mentioned provided the pharmacodynamics proof forJDT, and investigated the cural mechanism of JDT. The result showed that JDT hadan utility therapeutical effect on CPID model rat induced by phenol glue, had a goodantiinflammatory and analgesic effect, The results also showed that JDT was moreeffective than JC as a positive control. The therapeutical effect of JDT became betteras the dosage increasing. The mice didn't show the acute toxicity response, whichindicated that JDT was safty. To summarize, JDT is a new and promising dosage formfor the clinical treatment of CPID to develop.