Hemangiopoietin Supports Animal Survival And Accelerates Hematopoietic Recovery Of Chemotherapy-suppressed Mice

Objective. To determine if reconbinant hemangiopoietin(HAPO), a novelgrowth factor for primitive cells of hematopoietic and endothelial celllineages, accelerates hematopoietic reconstitution after high dosechemotherapy in vivo in mice.Methods. Male Balb/C mice after treatment of 5-flourouracil weresubcutaneously injected with HAPO or its dilution for consecutive 10days. Their survival and body weight together with peripheral blood wereroutinely tested. At day 7 and 14, the numbers of bone marrow cells aswell as colony-forming units(CFU) ater in vitro colony culture werecounted. The peripheral blood CFU and thd percentage of CD34~+CD117~+cells in bone marrow were analyzed. Transwell chamber was used for cellmigratory assay.Results. HAPO at different doses significantly increased the survivalrate and body weight, with an optimal effect in HAPO 10μg/day group.The number of bone marrow cells and the percentage ofCD34~+CD117~+cells were also increased after HAPO administration. Thenumber of CFU-GM and CFU-Mix in bone marrow after HAPOtreatment was greater than that from HAPO dilution group. Morecirculating CFU could be observed after injection of HAPO. In addition,this novel cytokine had a chemotactic effect on the hematopoieticstem/progenitor cells.Conclusion HAPO improves animal survival and accelerateshematopoietic reconstitution of mice after high dose chemotherapy.