| In the present work we use HPLC-UV method for determi-nation of Emodin and Physcion in Polygonum cuspidatum Sieb. etZucc.,aiming to control the medical herb quality. We useHPLC-UV method for determination of Emodin in rat plasma andliver after i.g administration of Polygonum cuspidatum Sieb.et Zucc. extract and study its pharmacokinetics. In this thesisthe study on determination and on pharmacokinetics of bioactivecompound of PoJygonum cuspidatum Sieb. et Zucc. is to providescientific evidence for the quality control and for how itsbioactive compound work.We develop a process and a HPLC-UV method for determinationof Emodin and Physcion in Polygonum Cuspidafun Sieb et Zucc.The equation of linear regression of Emodin: Y=3223308X+743.59, r=0.9999, linear range: 0.005～0.5μg. The LOD is0.04ng. The equation of linear regression of Physcion: Y=3525225X—3450.2, r=0.9999, linear range: 0.0038μg～0.38μg.The LOD is: 0.06ng. The average of recoveries is 100.6％,97.8％, 100.9％and 99.5％for free emodin, free physcion, totalEmodin and total Physcion, respectively.The method is reliable to analyse PoJygonum cuspidatumSieb.et Zucc. from 5 different habitates. The results show that Polygonum cuspidatum Sieb. et Zucc. from Yunnan has largestcontent of Emodin, that from Jiangsu has largest content ofPhyscion, and that from Gansu has smallest content both ofEmodin and Physcion.Compare the new method and the method of ChinesePharmacopoeia (2005). By means of HPLC to investigate theeffect of different processing method of Polygonum cuspidatumSieb. et Zucc. on emodin and physcion determination. Thecontents by new method are 10％higher for emodin and 20％higherfor physcion than those by Chinese Pharmacopoeia (2005).A reliable HPLC-UV method is developed to determine Emodinin rat plasma. The internal standard(IS) is 1,8-dihydroxyan-thraquinone, The equation of linear regression of Emodin in ratplasma:Y=0.7482X-0.065, r=0.9989, linear range: 0.2～6μg/ml.The LOD is 0.02μg/ml. The absolute recovery is 95.9～108. 1％.Sample preparation is achieved by liquid-liquid extraction.After i.g administrated the extract of Poiygonum cuspida-tum Sieb. et Zucc. to wister rats, the dose is 57.1mg/kg(calculated by Emodin),Tmax and Cmax is lOmin and 3.973±0.535μg/ml, respectively. Its pharmacokinetics parameters arecalculated by 3P97 software. The results show that in this dose,Emodin in Polygonum cuspidatum Sieb. et Zucc. extract is absorbed into blood quickly, the pharmacokinetic model ofEmodin fits two-compartment model in rat.Emodin concentration in rat plasma -time curve show twopeaks. The probably reasons are: hepatoenteral circulation orCuspidatin is absorbed slowly in rat. The concrete reason needfurther study.A HPLC-UV method is developed to determine Emodin in ratliver. The equation of linear regression of Emodin in rat liver:Y=0.1071X-0.0039, r=0.9995, linear range: 0.4667～39.22g/g. The LOD is 0.1924μg/g. The absolute recovery is84.4～101.2％. The internal standard(IS) is 1,8-dihydroxyanthr-aquinone and sample preparation is achieved by liquid-liquidextraction also. Determine Emodin in rat liver use developedmethod, Tmax and Cmax is 10min and 9.654±1.41μg/g, respectively.The results show that Emodin in Polygonum cuspidatum Sieb. etZucc. extract distributs over rat liver quickly, and isdisposed of completely throughout 24h, thus, Emodin would notaccumulate in liver.We take the bioactive compound of Polygonum cuspidatum Sieb.et Zucc. for the point of the study,and develop the study intwo aspects, one is study on quality of Polygonum cuspidatumSieb. et Zucc.,the other is study on pharmacokinetics and distribution in liver of Emodin after i.g administration ofPolygonum cuspidatum Sieb. et Zucc. extract in rats. The twoparts can show scientific evidence for the quality control andfor how its bioactive compound work, and reflect the entirestatus of bioactive foundation in the medical herb.
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