Research On The Effect Of MMPs And TIMPs On The Traumatic PVR

PURPOSES: To investigate the expression of MMP-2,MMP-9 and TIMP-1,TIMP-2 during the course of traumatic PVR treated with GM6001 and without GM6001, to explore the potential role of MMP-2,MMP-9 and TIMP-1,TIMP-2 during the course of traumatic PVR and to evaluate the effect of GM6001 on traumatic PVR prevention and treatment.METHODS: 360 SD rats were divided randomly into three groups: normal control group, the traumatic PVR group, the traumatic PVR treated with GM6001 group. The normal control group was intravitreous injected with normal saline. The traumatic PVR group was intravitreous injected with the PRP. The traumatic PVR treated with GM6001 group was intravitreous injected with the PRP and GM6001. The expression of MMP-2,MMP-9 and TIMP-1,TIMP-2 were qualitative and semiquantitative analysised with immunohistochemistry and RT-PCR on day 1, 3, 7, 14, 21 and 28.RESULTS:1. Immunohistochemistry showed that the expression of MMP-2,MMP-9 and TIMP-1,TIMP-2 were mainly located in the photoreceptor cells layer, out plexiform layer, inner plexiform layer and nerve fiber layer.2. The expression of MMP-2 and MMP-9 in the normal group and the traumatic PVR treated with GM6001 group were weak at all time. The expression of MMP-9 in the traumatic PVR group was strong at day 1, 3 and 7. The differences were statistical significance as compared with the normal group and the traumatic PVR treated with GM6001 group (P<0.01). The expression of MMP-9 in the traumatic PVR group decreased at the following time. The expression of MMP-2 in the traumatic PVR group was strong at day 14, 21and 28. The differences were statistical significance as compared with the normal group and the traumatic PVR treated with GM6001 group (P<0.01). The expression of MMP-2 in the traumatic PVR group decreased at the following time. The expression of TIMP-1 and TIMP-2 in the traumatic PVR group and the traumatic PVR treated with GM6001 group were strong at all time. The differences were statistical significance as compared with the normal group (P<0.01).3. The rate of MMP-9/TIMP-1 in the traumatic PVR group increased at day 1, 3 and 7. The differences were statistical significance as compared with the normal group and the traumatic PVR treated with GM6001 group (P<0.05). The rate of MMP-2/TIMP-2 in the traumatic PVR group increased at day 14, 21 and 28. The differences were statistical significance as compared with the normal group and the traumatic PVR treated with GM6001 group (P<0.05).4. RT-PCR showed that the expression of MMP-2,MMP-9 and TIMP-1,TIMP-2 mRNA and the rate of MMP-9/TIMP-1 and MMP-2/TIMP-2 in parallel with Immunohistochemistry showed.CONCLUSION: MMP-2,MMP-9 and TIMP-1,TIMP-2 involved in the course of traumatic PVR. The higher rate of MMP-9/TIMP-1 and MMP-2/TIMP-2 promoted the course of traumatic PVR. GM6001 played an important role in the course of traumatic PVR prevention and treatment by regulating the rate of MMP-9/TIMP-1 and MMP-2/TIMP-2.