The Research Of Interleukin 16 Cellular Resources In The Pleural Effusion.

Objective We discovered that interleukin 16(IL-16)could be detected in the pleural effusion in our prophase research. This research was to investigate the cellular resources of IL-16 in the pleural effusion.Methods We applied the method of cell culture to purify the mesothelial cells in the pleural effusion, the Wright's staining method to count the purity of the cultured cells and the method of immunocytochemical stain to identify the cultured cells. Then, we detected the intracellular IL-16 expression in the pleural effusion cells by flow cytometry(FCM), which, on the single cellular level, the surface antigen on the cellular membrane and the intracellular IL-16 were stained by the correspondent antibodies. Meanwhile, the different cells in the pleural effusion were separated by magnetic cell sorting (MCAS). Then, the cells separated were cultured in RPMI1640 with 10% FCS. At last, we extracted the supernatant after 48 hours and detected the concentration of IL-16 in the supernatant by ELISA.Results The percentages of CD3~+CD8~-cell, CD3~+CD8~+cell, CD19~+cell~+, CD14~+cell and PMC which can secrete IL-16 respectively were (71.93±17.73)% (n=34), (69.17±19.04)% (n=34), (45.30±18.77)% (n=15), (18.10±15.87)% (n=15) and (2.05±1.85)% (n=7). Between the non-malignant group and the malignant group, the percentages of CD3~+ CD8~-cell and CD3~+ CD8~+ cell which can secret IL-16 have significant difference(p<0.05). The concentration of IL-16 in the supernatant from the cultured cells, which were CD4~+ lymphocytes, CD8~+ lymphocytes, CD19~+ lymphocytes, CD14~+ cells and pleural mesothelial cells, respectively were (102.55±42.51) ng/L(n=5),(92.58±18.34) ng/L (n=5), (79.85±5.62) ng/L (n=5), (58.51±25.38) ng/L (n=5) and (18.1±8.37) ng/L (n=5).Conclusion IL-16 in the pleural effusion is mainly secreted by T lymphocytes, including CD3~+ CD8~- lymphocytes and CD3~+ CD8~+ lymphocytes. Secondly, B lymphocytes (CD19~+ cells) and monocytes/macrophages (CD14~+ cells )cansecrete partly, but pleural mesothelial cells almost can not secrete. The concentration of IL-16 in the pleural effusion is possibly related to the type and number of the cells.