Studies On The Chemical Composition In The Taxus Canadensis Marsh

Taxus is widely known as one of the excellent drugs within cancer treatment in the latest 30 years. Because taxol's content really micro in Chinese yew.therefore seeks taxanes compounds that can replace or may transform as the taxol hasthe necessity. Moreover, in chemistry domain along with taxanes compound medicinesource increasing, gradually regarding taxanes compound research complete synthesisby the natural products into effectively, the tolerance is better, absorbs a easier newgeneration of synonym, seeks has the better pharmacological action and the more widespread clinical practice derivative. Therefore, current besides protection wild Chineseyew resources and enhance theartificial nurture ability, pays great attention is to research not natural gain method for taxol. At present taxol is obtained by extraction andseparation,the cell raise and carries on half synthesis from baccatinⅢor10-deacetylbaccatinⅢof methods. Therefore,Seeks can synthesize and transform taxol's origin toappear importantly.This experiment mainly is a method which unifies using the positivephase chromatograph and the opposition chromatograph, conducts the research to in the Taxus Canadensis Marsh's matter. This article altogether is divided six chapters: First chapter willbe the understanding related Chinese yew's history, the development present situationand the future development direction, will perform to the Chinese yew to summarize;Second chapter carrys on preliminaryly qualitative to the sample through, the thin layerchromatographic analysis comparison and the highly performance liquid chromatography empirical value,and according to the preliminary extrapolation, provides the referencefor the design sample separation flow; The third chapter is the positive phase silicagel column chromatograph to the sample separation situation. This part altogetherseparates 7 compounds, has appraised 5 compounds; The fourth chapter is that theopposition chromatograph chromatographic separates and purifies the components. Thispart mainly carries on the separation and purification to taxol and cephalomannine:The fifth chapter is the taxol which produces mellowly carries on the solvent remnantallowance the determination.The result indicates the normal hexane and the acetoneremnant allowance conforms to "ICH" safety margin. The sixth chapter is experimentalsummary.This experiment altogether obtains 9 compounds.which has appraised 7compounds.4compounds belong to the taxanes diterpenes kind of compound. This separation purifi cation technology has been applied to the taxol industrialization production.This article innovates the spot:1:separate and obtain 3-(1′, 3′-di-hydroxyacetoneyl)-butyrolactone.2.This experiment uses the positive phase silica gel chromatograph union oppositionchromatographic analysis separation purification method maybe creation more valuesfor the industrialization production taxol and its analogs.3.This experiment the method which uses the positive phase silica gel chromatographunion opposition chromatographic method for the first time in the Canadian Chineseyew's taxanes compounds. Applies for the first time the JTY-1 opposition resin to theseparation purifies in the Canadian Chinese yew the taxusalkanes compound.