Immune Reactivities Determined By ELISPOT In Kidney Transplant Recipients And Their Clinical Relevance

To determine the immune reactivities in kidney transplant recipients and investigate their correlation with clinical events. Methods: To measure the frequency of donor reactive IFN-γproducing cells and TGF-βproducing cells using ELISPOT with recipients PBLs as the responders and donor splenocytes as the stimulators. Clinical events, including acute rejection and kidney graft function at 6m post transplant were correlated to ELISPOT readouts. Results: Donor reactive IFN-γELISPOT was associated with both acute rejection and graft function 6m post transplant, while no such correlation was found between TGF-βELISPOT and clinical events.Conclusion: Donor reactive IFN-γELISPOT can serve as a novel tool to further improve matching a recipient with a potential donor, excluding presensitized donor- recipients pairs. IFN-γELISPOT also appeared to correlate with kidney graft function at 6m post transplant, thereby carrying a potential to guide individualized immunosuppressive therapy.