| Objective: QDs as a new nanometer material, for its unique characteristic of action spectrum and stability of actinochemistry provides the possibility of imaging of viable cell and long-term observation of molecule in viable cell. But the question is whether the carcinoma cells marked by quantum dots(QDs) effect their biological behaviour. And that's the key of visible study of viable carcinoma cell. This project is to observe the growth invasion and metastasis of squamous carcinoma of tongue cell line Tca8113 marked by QDs and is to provide some evidence for the in-situ study of viable carcinoma cell using QDs.Methods: First, the colour, intensity and morpha lightening of QDs were observed under the CM. The Tca8113 cell lines were inoculated in 24-holes microtest plate and QDs were added into this plate after the cell attached to the wall of plate for co-culturing. Then the QDs entering into the cells were observed under FM. The different high concentration of QDs (0.1uMol,1uMol,2uMol) were added to eugonic Tca8113 cells. And then we observed the growth of Tca8113 and drew its growth curve. The methods of detecting the influence of QDs to the biological behaviour of Tca8113 includes: the experiment of cell invasiveness and basal membrane reconstruction done in transwell room; the average number of transmembrane cells representing the invasive ability of carcinoma cell; collecting non-adherent cell after washing by Hank's liquid for two times; getting average value of three random holes every point of time, and then calculating the rate of adhesion at different time. The average value of trans-membrane cell represents the chemotactic motion ablitity of carcinoma cell.Results: The QDs are red ellipse fluorophore, whose longest wavelength is 605 nm and strength of fluorescence is 250. After adding the QDs 40 minutes, they began to enter into the cell. 80 minutes later, there were many QDs, 120 minutes later, almost all the QDs were in the cell which located in the plasma of the cell. The growth curve of Tca8113 cells marked by different concentration of QDs and the contrast group are almost the same. The average values of trans-membrane cell marked by QDs and that not marked by QDs were 74.6±3.2, 76.5±2.8; which were not apparent difference. The rate of adhesion of cells marked by QDs and that not marked by QDs didn't have any significant difference, which indicates that the adhesive ability of Tca8113 marked by QDs has not changed. The number of cells marked by QDs and that not marked by QDs invasiving under the membrane were 83.8±4.1 and 85.4±3.7, which were not significantly different (P﹥0.05). That indicates that the chemotactic motion of cells marked by QDs is not changed.Conclusion: QDs can enter into the cell by internalization, and then can photo the cell. So it can be used to mark the surface and inner of the cell, and provide the basis of imaging the marker in viable cell. The QDs are red ellipse fluorophore, whose longest wavelength is 605 nm and the legth of fluorescence is 250. The QDs is located in the plasma of the cell. Different concentration of QDs (0.1uMol,1uMol,2uMol) has no affection to the growth of Tca8113 and so to the ability of invasiveness and chemotactic motion. The rates of adhesion of Tca8113/QDs and Tca8113 at the same time(30min,60min,90min,120min) have no significant difference. This study indicates that Tca8113 can be marked by high density of QDs and the QDs have no affection to the invasive adhesive and chemotactic ability of carcinoma cell. The study also provides the scientific evidence of research the viable cells and their molecules in physical condition based on the QDs.
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