Expression Of Beta-Catenin And GSK-3beta In Hypoxic Rat Embryo Brain And The Relationship Between HIBD And Wnt Pathway

Part I Establishment of Intrauterine Hypoxic-ischemicAnimal ModelObjective:To establish animal model of intrauterine Hypoxia-ischemia in rat by clamping uterine and ovarian arteries for 15 minutes.Methods:Uterine arteries of the pregnant rats were clamped for 15 min and reperfused for 24 hours after clamped. Pathological changes of brain were observed with light microscopy and brain cell apoptosis detected by TUNEL kit.Results: Embryonic cerebral ischemia-reperfusion injury has been observed. Cerebral neuron apoptosis in ischemic brain significantly increased. White matter damage was the most obvious at all.Conclusion: H/I fetal brain injury can be induced by clamping uterine arteries of the pregnant rats. Part II GSK-3beta and beta-Catenin Expression in EmbryonicBrain with Hypoxic-ischemic DamageObjectives:To detect expression of Beta-Catenin and GSK-3beta in embryonic brain with intrauterine ischemia and explore the relationship between the hypoxic- ischemic brain damage and Wnt signaling pathway.Methods: MRNA expression of GSK-3beta and beta-Catenin in embryonic brain of intrauterine hypoxia-ischemia model were detected by fluorescence quantitative PCR and protein changes of GSK-3beta and beta-Catenin were checked with western blot. Two-factor analysis of variance was used to analyze the results.Results: The differences of Beta-Catenin and GSK-3beta in mRNA expression between normal group and ischemic group were not significant. Protein expression of them in ischemia group was higher than that in control group.Conclusion: Wnt signaling pathway may be involved in hypoxia-ischemia brain damage. The nodes may be in post-transcriptional translation stage.