| Objective:The purposes of the researching here were to make the research of theeffects and safety of Leonurine injection on uterine contraction andhemostatic effects in intra- and post-cesarean section delivery, and observethe effects of Leonurine injection on the tension, intension, amplitude andrat of myometrium contraction in order to illuminate the mechanism. Andthe aims of the researching are to provide the effective, safe and affluenttraditional Chinese medical preparation for parturient and patients with PPHto improve the modern research of the traditional Chinese medicine.Methods:From January to September 2006, 278 cases who received cesareansection delivery were collected and analyzed. The gestational weeks werecarefully checked by last menstrual period, fetal movement and ultrasound.The cases, with the exception of the vaginal delivery, less than 28 weeksand unwilling to use Leonurus injection, were divided into two groups:Leonurus group which were treated with Leonurus injection, and the groupwith oxytocin. The clinical observation indexes included bleeding volumes after the operation for 2～72 hours, uterus recovery, function of bloodcoagulation and untoward reaction. Meanwhile, the human myometriumwhich obtained in CSD were dissected into two myometrial strips andsuspended in 20ml organ bath in uterine solution under isometric conditions.Undergoing spontaneous contractions, myometrium were exposed toleonurous injection and oxytocin in the dose range of 0.5ml to 1 ml. The rat,tension, intension and amplitude of myometrial contraction were measured.Result:The bleeding volumes intra- and post-operation in Leonurus group was279.44±151.77 ml. There were no significant differences from those inoxytocin group(292.85±168.25ml) (P＞0.05) The hemorrhagic volume about2 hours after the operation in leonurus group was 52.49±55.20 ml and therewas no difference with oxytocine group (54.30±72.39 ml)(P＞0.05). Thebleeding volume of 2 to 72 hours after operation showed no differencebetween the two groups (P＞0.05).The cases of hemorrhagic volume more than 400ml were 27.2% and30.3% respectively from the operation to 2 hours later and were 20.6% and26.8% respectively to 24 hours later which the volume was more than 500ml. There was no significant difference in the incidence of postpartumhemorrhage between Leonurus group (20.6%) and oxytocin group (26.8%)(P＞0.05). No difference was demonstrated in the extra addition ofuterotonics during two groups (P＞0.05). The time of contraction after using uterine injection was as follows:2.61±1.05 minutes in the leonurus group and 2.40±0.83 minutes in theoxytocin group (P＞0.05).There was no change in the function of blood coagulation in theleonurus group. And no untoward reaction has been observed in the test.After exposure to a dose of 0.5ml leonurus, the uterine myometriumcontracted strongly. There was an apparent potential in the rate, tension,intension and amplitude of contractions. But two groups had no difference(P＞0.05). The contractive amplitude raised from 441.3±205.0 mg to897.6±264.2 mg after the exposure to 0.5mg/ml leonurus injection, andthere were obviously differences between two groups. The contractileamplitude was measured the increasing from 897.6±264.2 nag in 0.5mg/mlto 1466.53±401.0 mg in 1mg/ml leonurus injection, which had obviouslydisparity (P＜0.05). After increas the dose of injection, the tension, intensionof the uterine contraction seemed strongly while the rate showed smoothly,which have no differences between two density.Conclusion:The administration of leonurus injection intra-and post-operation mayimprove uterine contractility and have the effect on hemostasis intra- andpost-operation. And Leonurus injection can improve the uterine contractilityboth in vivo and ex vitro.
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