The Studying About Functional Reconstruction Of The Traumatic Third Cranial Nerve

Partâ… : To establish the rat' s oculomotor nerve animal model in whichthe nerve was lesioned and repaired in different positions and ways.Objection: To establish the rat's oculomotor nerve animal model inwhich the nerve was lesioned and repaired in different positions and ways.Methods and materials: ninety S-D rats, during the operation an enlarginginfratemporal fossa approach was used, through it the third cranial nervewas discovered, the oculomotor was dissected in post cavernous sinus,cavernous and orbital opex, then approached the nerve stumps; incavernous sinus the nerve dissected, then repaired the lesioned nerveas suturing, fibrin glue adhering, fixing up the nerve stumps with plasmaclot; after suturing the lesioned nerve in cavernous, the NGF wasinjected into extraocular muscle, results: the anatomy of the oculomotornerve was obtained, postoperation, the mortality rate was 5%, the pupildisffused and extraocular muscle paralysised in all rats, some rats wereobserved somewhat disable. conclusion: the animal model is security,efficiency, credibility, it can be used to the study of the functionalreconstruction of the lesioned oculomotor.Partâ…¡: To investigate the relationship between the distance from thetraumatic position on the third cranial nerve to the target organ andregeneration and functional recovery of posttraumatic oculomotor nerve.Objection: To investigate the relationship between the distance fromthe traumatic position on the third cranial nerve to the target organ andregeneration and functional recovery of posttraumatic oculomotor nerve.Methods and materials: Thirty male Sprague-Dawley rats were separated tothree groups, the rats were undergo craniotomy and microsurgery throughright subtemporal approach and the oculomotor nerve were dissected incavernous sinus, post cavernous sinus and orbital opex, then approachedthe nerve stumps, the anatomy and histology of the oculomotor nerve werestudied after operation and the functional recovery of the regeneratednerve was observed. Results: anatomical demonstration of nonselectivereinnervation was obtained by injecting horseradish peroxidase (HRP) intothe extraocular muscles, the number of somatic motility neurons indiencephalon was uniformity, the original relationship between musclefibrins and their innervating neurons In which the nerve dissected inorbital opex was better than the rats which nerve dissected in post cavernous sinus and cavernous sinus, the degree of the functionalrecovery of extraocular muscle as well.Conclusion: the nearer thetraumatic position to the target organ, the better the functional recoverywas, probably the key factors was the degree regenerated nerve fibrininnervated the extraocular organizing.Partâ…¢: To investigate the different means in repairing the lesionedoculomotor and their effect on functional recovery and nerveregeneration.Objection: to investigate the different means in repairing thelesioned oculomotor and their effect on functional recovery and nerveregeneration. Methods and materials: 30 male S-D rats were separated tothree groups, their oculomotor was dissected in cavernous sinus andrepaired the lesioned nerve as suturing, fibrin glue adhering, fixing upthe nerve stumps with plasma clot. the anatomy andhistology of theoculomotor nerve were studied after operation and the functional recoveryof the regenerated nerve was observed. Results: The functional recoveryin the groups which the nerve stumps sutured and adhered with plasma clotwere better than the groups nerve stumps adhered with fibrin glue. In thefirst groups, the number of somatic motility neurons in diencephalon wasmore than the other groups, and the regenerating nerve fibrin organizedwell. Conclusion: Probably the methods which approached the nerve stumpsby suturing and plasma clot were better than the method adhering the nervestumps with glue fibrin, the key factor affecting the functional recoverywas the stability between the adhering nerve stumps, and decreasing thescar of it, the degree of the functional recovery was determined by wellorganized regenerating nerve fibrin reinnervating the extraocular muscle.Partâ…£: To investigate the effect of the explicit NerveGrowth Factor(NGF) on the lesioned third cranial nerve.Objection: to investigate the effect of the explicit Nerve GrowthFactor(NGF) in the lesioned third cranial nerve. Methods and materials:20 S-D rats were separated to two groups, dissected the third cranial incavernous sinus and sutured the nerve stumps, after operation injectedNGF in extraocular muscle everyday in 10 rats, the other group did notgive NGF, and the nerve fibrin organized well as well. Results: accordingto the postoperative demonstration, the regenerated nerve fibrin in therats injected NGF were more than the rats without NGF. Conclusion: NGFcould promote the growth of the traumatic nerve and functional recovery.