Expression Of Inhibitor Kappa B-alpha In Experimental Acute Pancreatitis And Effect Of Qing Yi Tong

Objective To study the expression of inhibitor kappa B alpha (IκBa) in thepancreas and liver tissues of rats with experimental acute pancreatitis (AP), and toexplore the therapeutic mechanism of Chinese medicine Qing Yi Tong (QYT) on AP.Methods 70 SD rats were randomized divided into three groups: normal controlgroup(n=10), AP+QYT group(n=30), and AP+normal solution (NS) group(n=30).AP model was induced by retrograded injection of 4ï¼…sodium deoxycholate into thepancreatic duct. QYT or NS were infused to the rats respectively by a gastric catheter,and which was repeated every five hours after AP induction. At 1h, 4h, 10h afteroperation, rats were sacrificed individually. The samples of serum, pancreas, liver,and lung tissues were removed out for the further tests and examinations. Real-Timereversed transcriptional-polymerase chain reaction (RT-PCR) was performed todetect IκBa mRNA expression in the tissue of liver. Western blot was taken todetect IκBa protein expression in the tissues of liver and pancreas. IκBa proteinincluding phosphorylated form (IκBa-p) and non-phosphorylated form (IκBa-n) wastestd. Serum level of leukriene C₄(LTC₄) was detected by enzyme-labeledimmunosorbent assay (ELISA). The pathological changes of pancreas and lungtissues were stained by HE and observed under light microscope.Results Compared with the normal control group, expression of IκBa mRNA inliver was higher in AP rats (P＜0.01,or P＜0.05). QYT treated group had lowerexpression of IκBa mRNA in liver as compared with AP+NS group (P＜0.05).Expression of IκBa protein (including IκBa-p and IκBa-n) in liver and pancreaswere also higher in AP group as compared with that in control group (P＜0.05);IκBa-p displayed an increased tendency during the observation period in AP+ NSgroup. However, QYT treatment induced a decrease of IκBa-p proteinexpression, and an increase of IκBa-n expression.The serum LTC₄ level in AP groupwas increased with a time-dependent manner, QYT attenuated the increased LTC₄level in certain degree (P＜0.05). In pancreas and lung tissues of AP rats, interstitialedema, hemorrhage, and considerable inflammatory cells infiltration were foundwith a pathological score of 9,12,13 in 1h, 4h and 10h, respectively. These changeswere lightly ameliorated by OYT intervention.Conclusions In the study, the rats with acute pancreatitis displayed pathologicalchanges in multiorgans and a serious inflammatory reaction. Expression of IκBa both in the mRNA level and protein level was increased 1h after AP induction andcontinued to rise during the observation time of 10h in the experimental AP. Theincreased IκBa protein was mainly the IκB-p form, implying nuclear factor-kappaB(NF-kappa B) was activated at early stage of AP, and which may contribute to thesystemic inflammatory response in AP. QYT ameliorate the inflammatory reaction ofAP by inhibiting IκBa-p expression and then reducing inflammatory mediator.