| Objective:Toll-like receptor(TLR) that recognize microbial pathogens play a critical role in innate immunity;however,their expression and function after surgery remain unknown.The aim of this study was to examine TLR2 and TLR4 expression on monocytes in infants and neonates undergoing major surgery.Methods:The subjects were 30 patients which included 9 neonates,10 infants and 11children.Blood samples in neonates were obtained before the operation,at the end of the operation,and 1, 3 days after the operation; blood samples in infants and children were obtained before the operation,at the end of the operation,and 1, 3 ,7 days after the operation. TLR2 and TLR4 expressions on peripheral blood mononuclear cells(PBMCs) were analyzed by flow cytometry.Results: A significantly decreased frequency of TLR2 and TLR4 in infants and children were observed at the end of the operation (P<0.05).And then recoverd to the preoperative values on postoperative day 1 (P>0.05). TLR2 in neonates showed the same changes with infants and children; TLR4 had no significant change compared with preoperative values(P>0.05).Conclusion:expression of TLR on monocytes in infants and neonates undergoing major surgery were downregulated by operation,which can suppress immune responses and increase the risk of infection.The present study may offer new insights for postoperative modulation of innate immunity under surgical stress.
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