Experimental Study On Effect Of Hypertonic-hyperonconic Solution On Expression Of Rabbit MRNA Of TNF-α,IL-6 And IL-10 Following Hemorrhagic Shock

Objective:To study the effect of hypertonic - hyperonconic solution on expression of rabbit mRNA of TNF-α,IL-6 and IL-10 following hemorrhagic shock and discuss its effect on prevention and treatment of ALI.Methods: 40 newzealand rabbits were divided into experimental group(HH40 group)and control group(LR solution)by random,each group consisted of 20 rabbits. A model of rabbit with hemorrhagic shock was established by Wigger's revised method. Then,in 60 minutes the animals were resuscitated with HH40 solution (5ml/kg,ivgtt)respectively , which are as 1.5 times as hemorrhagic volumes, subsequently,the two groups animals were infused the half of hemorrhagic volumes back to their bodies. Electrolyte and arterial blood gas were measured before shock,during shock before resuscitation, and after resuscitation in 1hour and 4h. which peripheral mononuclear cells were isolated to measure the expressions of TNF-α,IL-6,IL-10mRNA in circulation blood after resuscitation in 1h and 4h .The animals were killed with gas embolism after blood sample were collected. Lung tissues were taken to measure expressions of TNF-α,IL-6,IL-10mRNA,ratio of lung wet and dry weight and investigated on histopathology.Results:HH40 could make MAP of shock rabbit elevate significantly in a short time and arterial partial pressure of oxygen return gently, the tendency of decline was stepped down on blood pH,buffer excess(BE),standard bicarbonate (SB), moreover, after administration blood sodium ion rose insignificantly ;lung pathologic change was relieved contrast with LR group,the expressions of local TNF-αand IL-6 mRNA was obviously lower than those of LR group, but IL-10 mRNA was higher;And in circulation blood the expressions of TNF-α,IL-6,IL-10mRNA were significant in 1h and 4h ,but not obvious difference beteen two groups.Conclusion: hypertonic-hyperonconic solution can maintain hemodynamic stability more efficiently and persistently, improving hemoperfusion local organ ,inhibitting synthesis and expression of proinflammatory factor, up-regulating synthesis and expression of anti-inflammatory factor, and restore the balance among cytokine networks.