Experimental Study On The Depressant Effect Of Sodium Arsenite On Human Stomach Carcinoma Cell SGC-7901

Recent years have witnessed further researches and abundant documents on the therapy of hematological system tumors by utilizing arsenical but poor reports relevant of studies on solid tumors from around the world and the research directions didn't deepen the exploitation of mechanism of action of the drug and were merely confined within aspects such as clinically therapeutic effects and biologically behavioral effects. In the experiment, we observed the effect of sodium arsenite on the expression for transcribed mRNAs and translated proteins of apoptotic gene Bax and apoptotic inhibition gene Bcl-2 by using the research object , human stomach carcinoma cell SGC - 7901 cultivated in vitro in order to reveal its mechanism of action.Objectives To approach the mechanism of action of sodium arsenite(NaAsO2) on human stomach carcinoma cell SGC-7901 to reveal the relationship between the mechanism and apoptotic gene Bax and apoptotic inhibition gene Bcl-2. Methods After having divided cell SGC-7901 in logarithmic growth phase into 3 groups, blank contrast group, cell contrast group and experimentally contrast group and had sodium arsenite of 5.0umol/L,10.0umol/L and 20.0umol/L function against cell SGC-7901 after in defferent time, we have determined the value of absorbance at 570nm wave length and detected value A by MTT colorimetry to calculate cell inhibition ratio, to analyze cell cycle by flow cytometry and the distribution of cell cycle by Modfit LT2.0 software and to detect the effect of the drug on the expression for mRNAs of Bax and Bcl-2 by ICC and RT-PCR.Results⑴There was a concentration and time dependence between sodium arsenite and the corresponding cell growth inhibition. The cell inhibition ratio ascended with the accrescence of drug concentration and prolongation of action time and the disparity embodied statistical significance(P<0.05).⑵The cell growth was retarded at three stages G1,G2 and S of which the retardarce at G2 stage was most significant after sodium arsenite of different concentration had functioned for 48h(P<0.05);there appeared an apoptotic peak prior to G1 peak illustrated at cell cycle histogram.⑶Immunocytochemical methods illustrated that the expression of the protein of Bax obviously ascended in contrast with that of the contrast group cells while the expression of the protein of Bcl-2 obviously descended in contrast with that of the contrast group cells after sodium arsenite of different concentration had functioned for 48h.⑷RT - PCR demonstrated that the strap concentration of DNA of Bax accentuated obviously while that of Bcl-2 descended. Conclusion The results of MTT of this experiment demonstrated that sodium arsenite of variable concentration (5.0umol/L,10.0 umol/L,20.0umol/L)could uniformly and effectively inhibit the growth of SGC-7901 cell with time and concentration dependence. Sodium arsenite implemented growth inhibition and induced cell apoptosis of human stomach carcinoma cell SGC - 7901 to perform anticarcinoma presumably by up-regulating the expression of Bax and down-regulating the expression of Bcl-2.