Study On Diagnosis Of Urothelial Carcinoma Of Bladder By Multicolor Fluorescence In Situ Hybridization

Objectives To detect chromosomal aberrations in urothelial carcinoma (UC) of bladder and urine samples of the patients in order to find the optimal probe sets for diagnosis of the disease.Methods Fluorophore labeled probes to chromosomes 3, 7, 8, 11, 16, 17, Y centromeres and 9p21 were detected in 96 tumor tissues of bladder UC and 66 urine specimens. Positive results were defined according to UroVysion criteria. Resultsâ‘ The rates of chromosome aberrations were 67.7% (9p21), 64.5% (Y), 59.4% (17), 55.2% (7), 53.1% (8), 50.0% (3), 49.0% (16) and 42.7% (11) in cancer tissues, and 64.6% (Y), 59.1% (9p21), 57.5% (8), 56.1% (17), 50.0% (7), 50.0% (3), 46.9% (11) and 42.4% (16) in urine samples.â‘¡Aneuploidies of chromosomes 3, 7, 8, 11, 17 in both cancer tissues and urine specimens were associated with tumor stages (P<0.05). There was significant difference in abnormalities of chromosomes 3, 7, 8, 11, 16, 17 and Y in cancer tissues and chromosomes 7, 11 and 17 in urine specimens between G1-2 and G3 (P<0.05).â‘¢When according to UroVysion criteria, the best probe sets were 7, 8, 9p21, 17 (60.4%), 7, 9p21, 16, 17 (60.4%), 3, 7, 9p21, Y (61.8%, male) and 7, 8, 9p21, Y (61.8%, male) in cancer tissues, and 3, 7, 9p21, 16 (63.6%), 7, 9p21, 16, 17 (63.6%) and 7, 8, 9p21, Y (66.7%, male) in urine specimens. The common probe sets for the two groups of specimens were 7, 9p21, 16, 17 and 7, 8, 9p21, Y (male).Conclusion There existed frequent aberrations in tumorous tissues and exfoliated cells of bladder UC. The optimal probe sets in urine specimens for diagnosis of bladder UC might be 7, 9p21, 16, 17 and 7, 8, 9p21, Y (male).