Protective Effects Of Ginsenoside Rg1 On Renal Fibrosis In Subacute Aging Mice

Objective To induce KM mice by high dose of D-galactose and establish a subacute aging model, to investigate protective effects of Ginsenoside Rg1 on renal fibrosis in mimic aging mice.Method Male KM mice aged 3 months were induced by consecutively intraperitoneal injection of D-galactose (400mg/kg) for 12 weeks . The activities of superoxide dismutase (SOD) in kidney tissue were examined and SA-β-galactosidase activity were measured with histochemistry staining. Renal pathological changes were observed by microscope and transmission electron microscopy. So we established a model of subacute aging mice. Then 3-month-old Male KM mice were randomly divided into six groups( eight mice in each group): young group, model group, Ginsenoside Rg1 group(treated with some certain dosage of 5,10,20mg/kg/d) and losartan group(10mg/kg/d , positive control group,). Treatment lasted 12 weeks. Then we compared senescent indicators as above. Furthermore, Immunohistochemical studies were performed to detect the expression and distribution of FN,TGFβ1,βigh3 in the kidney of those mice on different groups. And the levels of TGF-β1,βigh3 were also investigated by Western-blot.Results Compared with young group, The activities of SOD in the kidney on model group were decreased. Positive staining for SA-β-galactosidase were widely observed. Significant structural changes, including tubular astrophy, dilation, intratubular casts, cellular infiltration, glomerlar sclerotic and mesangial matrix expansion were found through microscope and transmission electron microscopy. And the expression of FN,TGFβ1,βigh3 increased remarkably. Compared with model group, Ginsenoside Rg1 could improve all senescent indicators as above in some degree, and the group treated with the dosage of 20mg/kg/d showed the most effective protection. Meanwhile , the renal expression of FN,TGFβ1,βigh3 were reduced significantly in the Ginsenoside Rg1 group(20mg/kg/d). Compared with losartant guoup, Ginsenoside Rg1(20mg/kg/d) had the same effect of lessening the tululointerstitial damage(P > 0.05)., but on the reduction of mesangial matrix expansion , it was not as good as losartant(P<0.05).Conclusion Ginsenoside Rg1 may exert a renal protection on subacute aging model induced by D-galactose in mice.The probable mechanism may possibly partly relate to its effect of antioxidation and reduction of TGFβ1 andβigh3 expression.