Differentiation Of Human Adipose Derived Stem Cells Induced By Mesenchymal Stem Cells Transfected With Human Fibroblast Growth Factor 10

Objective: To investigate the differentiation of adipose derived stem cells (ADSCs) induced by human fibroblast growth factor 10 (FGF10) and to offer a novel method to increase subcutaneous adipose tissue in clinic.Methods: 1'Isolate, culture and in vivo expand ADSCs from human hypodermal resected adipose tissue, identify the ADSC by immuno-fluorescence, FACS and lineage-specific differentiation; 2'Human FGF10 cDNA was amplified by RT-PCR from total RNA of human lung tissue and was cloned into vector pcDNA3.0 which were transfected into MSC, the FGF10 expressed by pcDNA3.0-FGF10-MSC was confirmed by IHC, ELISA, RT-PCR and western-blotting; 3'Observe the figure of ADSC and detect the expression of transcriptional factor during the differentiation of ADSC co-cultured with pcDNA3.0-FGF10-MSC; co-inject pcDNA3.0-FGF10-MSC and ADSC into the subcutis of immunodeficient (SCID) mice.Results: 1'ADSCs possess relatively specific characters of being a stem cell line through identification and has a potential to be induced to many specific cell lineages; 2'pcDNA3.0-FGF10-MSC cell strain can express FGF10 continuously; 3'The fat droplets of ADSC became hypertrophy and hyperplasia; the transcriptional factor of ADSC increasing with the time of co-culture; adipose tissue of the experiment group mice more thicker than blank group, and the subcutis tissue displays brown colour by IHC of HLA-1.Conclusion: ADSCs was a stem cell line in this experiment and FGF10 can induce the differentiation of ADSC through co-culture in vitro and co-inject in vivo.