The Research On Screening Differentially Expressed Genes Of Guinea Pig With Noise Induced Hearing Loss Susceptibility

Noise induced hearing loss (NIHL) is an auditory disorder affecting widely the officers and soldiers in military noisy environment. This study has achieved the following purposes,observed effects of different levels of noise exposure on the auditory function and the hearing organ of guinea pigs,explored optimal noise exposure profiles for research of NIHL susceptibility,established NIHL susceptibility model,148 differentially expressed genes of cochlea between NIHL susceptible and unsusceptible guinea pigs were screened by using suppression subtractive hybridization.The results of bioinformatics analysis showed that there are 34 sequences whose homology were larger than 80% and match sequence larger than 100 bp. Results of preliminary analysis on some sequences show that the mitochondrial genome and the heat shock protein were correlated with the occurrence of NIHL and its susceptibility. The results of this study will provide foundation for further study on the pathogenesis of NIHL susceptibility.