Function Of Irf1 Gene In VSMC Proliferation Of Diabetic Angiopathy Rat With Hypertensions

To investigate the function of Irf1 gene on the high expression change of proliferation-related in vascular smooth muscle cells (VSMCs) of diabetic angiopathy rat with hypertension, discuss the mechanism of Irf1 gene result in proliferation is must possess important significance for prevention and therapy of vascular complication. In this study, Adopt plant block method foster SD and SHR rat VSMC, establish subsequence investigate cell model in vitro, adopt PCR etc methods clone Irf1 gene coding list, thus successfully established pcDNA3.1-Irf1 eukaryon expression carrier. During the examination of flow cytometry to identify the growth curve and PCNA immunity cell chymistry methods and MTT etc, we could discover, it has excessively indicated the gene swelled DNA compose even accelerate the course on VSMC cell cycle for proliferate of cell has visible function. Then combine the work we had been done prophase, principium improved the important function of Irf1 gene on the change of proliferation-related in vascular smooth muscle cells (VSMCs) of diabetic angiopathy rat with hypertension.