Experimental Study On Endothelial Progenitor Cells For Angiogenesis Of Lung Cancer Tube

Objective:To make use of endothelial progenitor cell's positive role on the process of angiogenesis. Methods:We study the angiogenesis of lung cancer. Endothelial progenitor cells were isolated from PBMC and then in vitro differentiate,culture and amplify for endothelial progenitor cells, To lable endothelial progenitor cells with DAPI fluorescence,To transplant both marked cells and lung carcinoma SPC-A1 cells in BALB/C mice meanwhile, and observe endothelial progenitor cells'effect on angiogenesis during tumor growth. Results:Endothelial progenitor cells can be obtained by culturing the peripheral blood and amplificated by about 100 times in vitro.Through subcutaneous inoculation,we get subcutaneous inoculation xenograft tumor mode of SPCA-1. Nude mice mean life of group A were 85.±9days,group B 78±8days.Gross tumor volume of group A were 1.44±0.45cm3, group B 1.83±0.32cm3。In the period of observation, The mean life span difference of three groups nude mice had sence(P<0.05). The gross tumor volume's difference between group A and group B had statistical significance(P<0.01).Conclusions:It is suggested that endothelial progenitor cells can participate lung cancer tube angiogenesis,hasten the growth of lung cancer and make life of nude mice shorter.