| Diabetic Retinopahty is the characteristic pathological change in the ophthalmopathy of diabetic, it often lead visual extinction and even blind. In the early stage,most experiments concentrated on the retinal vasculopathy in diabetes. Recent years more and more researchs discovered that it can detected the function change of retina before the retinal vasculopathy, it manifested that diabetes had the direct affection to nervous layer of retina and didn't occurrenced after the breakdown of blood-retina barrier, vasculopathy was one of the directions to cause the handicapped of retina.The new views think that the alteration of retinal neurons and neuroglia development before the vasculopathy, moreover the alteration of retinal neurons and neuroglia possible induce the retinal vasculopathy and macular edema.The breakdown of blood-retina barrier,hypoxia of retina in the early diabetes may cause the concentration of glutamate in retina increased, may provoke the retinal neurons such as retinal ganglial cells exitotoxicity damage and the death, thus cause deprivation of retina. But the exitotoxicity of glutamate will increase the oxidative stress further. Muller cell is the dominant glial cell to scavenge the excess glutamate out of the cell, the GLAST,GS,GD in Müller cell membrane are the several mainly component element to converying and degrade the glutamate. The function of Müller cell damaged in the early diabetes, which has the relationship with the increase of oxidative stress.But it stil didn't know how to alteration the function after it intake and degrade the glutamate, the really mechanism to breakdown the barrier of blood-retina.Taurine (diamidoethylsulfonic acid ) is one of theβ-amino acids in vivo, it belongs nonprotein amino acid, it mainly distribution in the tissues which have high excitability such as nervous system,muscle tissue,retina,lymphocyte and platelet, it has the important physiological functions in vivo. Taurine is abundance in photoreceptor cell and Müller cell, it relate to the growth and development of retina and maintenance normal visual function. The latest data demonstrate that taurine can inhibit the function of neuromodulator in retina and inhibit the excite toxic action of glutamate simultaneously.It displayed, in the early stage of diabetic retinopathy, the function of retina neuron and glial cell has disordered before the telangiosis in retina developed. It appearanced that the expression of Glial fibrillary acidic protein in Müller cell increased,the function of glutamate transporter disorderd and the subtype of glutamic acid receptor activated, but the contents of taurine in retina decreased gradually with the course of diabetes prolonged.On account of the above analysis, this experiment by means of culture the muller cell of rat retina in high gloucose as the model in vitro to reasearch the change of muller cell in diabetic Retinopathy.Using the TUNEL,immuocytochemistry,Western blotting,Real Time-PCR and so on technique methods after intervention by taurine, observing the influence of high glucose and taurine intervention to the expression of GFAP,GS,TAUT,S-100. Aim at to further research the influence and the possible molecule mechanism of taurine to retinopathy in early diabetes.Results and conclusions:1. In the retinal cells from SD rat which mix culture at archae-generation, the syngenesis of neurocyte and glial cell is good, the neurocyte is more sensitiveness to high glucose damage, the survival rate decreased, the survival rate can be incresed obviously by taurine.2. The cytoactive of muller cell in retina which cultured in high glucose can be descended severity and have many apoptosis cells , the cytoactive of muller cell can increased after intervention by 0.1mmol/L,1mmol/L,5mmol/L,10mmol/L taurine, and the number of apoptosis cells decreased obviously, it indicated that taurine can promoted the proliferation of Muller cell by means of dose-dependent and inhibited the apoptosis of Muller cell from retina which induced by high glucose.3. The damage of high glucose can cause the mRNA and protein expression of GFAP increased which is the characteristic protein of Muller cell from retina, the nuclear-cytoplasmic ratio expression of S-100 is decreased. 0.1mmol/L,1mmol/L,5mmol/L and 10mmol/L tautine can attenuate the high expression of GFAP which caused by high glucose. The nuclear-cytoplasmic ratio expression of S-100 is enhanced after treated by 0.1mmol/L,1mmol/L,5mmol/L and 10mmol/L taurine. It indicated that the cell function change of Muller cell induced by high glucose can been lessen or forbid by taurine.4. The expression of GS can been inhibited by high glucose, high glucose can cause the mRNA and protein expression of GS decreased. Taurine can inhibit the mRNA and protein expression of GS decreased which caused by high glucose, the expression of GS recover to normal level gradually after contribution by different concentration taurine.5. Taurine maintenanced the high concentration in cell by the transmembrane active transport of TAUT in cellular membrane, through the high glucose model of Muller cell which constructed in vitro, we observed that high glucose inhibited the transmembrane transport of Muller cell, the expression of TAUT up-regulated after supplied taurine.As the above review, high glucose damage can cause the change of morphous and chatacteristic protein of Muller cell from retina, after treated by taurine, the protective effection to Muller cell from retina which caused by high glucose damage is visible. This experiment have provide the experiment basis to use taurine protective the retina damage in early diabetes.
|
PDF Full Text Request