The Protective Effects Of Sodium Selenite And Aloin Against UV-induced Damage Of Dermal Fibroblasts

Objective:To study the influence of UV on the proliferation of dermal fibroblasts,the oxidative damage and collagen synthesis and to determine the protectiveeffects of sodium selenite and aloin against ultraviolet irradiation and thepossibility of their application in the prevention and treatment of photoaging.Methods:Human dermal fibroblasts in vitro were incubated with or withoutdifferent concentrations of sodium selenite or aloin for 2 hours, and thenirradiated by a single exposure to UVA (320-400nm) or UVB (280-320nm).Twenty-four hours later cell proliferation rate were measured by MTTassay;SOD activity, GSH-Px activity and the content of collagen protein weredetected by biochemical assay.Results:5J/cm~2UVA and 40mJ/cm~2UVB could inhibit the proliferation of thefibroblasts, decreased the activity of SOD and GSH-Px and collagensynthesis. Sodium selenite reversed the suppression of the cell proliferationby UVA or UVB in some degree(P＜0.05). Sodium selenite could enhancethe activity of the GSH-Px, which was dose dependent. Sodium seleniteenhanced the activity of SOD on the UVA irradiated fibroblasts at a concentration of 80μg/L. Collagen synthesis were markedly up-regulated bypre-treatment with different concentrations of sodium selenite(P＜0.05).10-100 mg/L aloin reduced the UV induced inhibitory action of cellproliferation. Aloin in dose-dependently increased the activity of the GSH-Px,SOD and the collagen synthesis.Conclusions:1. UV irradiation could inhibit the proliferation and antioxidant ability ofthe dermal fibroblasts, reduce the collagen synthesis.2. Sodium selenite and aloin had photoprotective properties aginst UVirradiation in vitro and may be used as anti-photoaging agents.