Research On Electroporation Transfection Chlamydia Trachomatis To Build Vector Of Chlamydia

Objective: In order to study the feasibility of electroporation introduction foreign DNA of plasmid into chlamydia trachomatis (CT)for transfection and approach new carrier system based on CT which can carry foreign DNA or plasmid into eucaryotic cell, to lay the fundation of Gene therapy.Methods: Electric pulses of intensity of 2.5 kilovolts and 1.5kilovolts per centimeter were put on the suspension of CT sero type D and the plasmid pECFP-C1/U6-2. After the electrotransformation we culture the CT in Hela cell, to proliferate the CT and the plasmid. At the same time culture the CT, we observe whether the green fluorescent protein(GFP) gene express in the CT or in the Hela cell. At last we amplificate the insertion sequences in pECFP-C1/U6-2 to test whether the plasmid were electrotransformed into CT.Results: The plasmid pECFP-C1/U6-2 were electrotransformed into CT under the 2.5 kilovolts electric pulses, and exist stably for several generations. We do not found GFP both in CT and in Hela cell.Conclusion: We build the model to transfer foreign gaint molecular substance into CT. Electrotransformation can transport foreign DNA into CT. We do not found evidence that plasmid with expressor and promoter of E.coli can express in CT.