| Objective Under multiple factors,nerve tissues suffer the attack of oxygen free radical after ischemia and lacking of oxygen,what may cause multiple kinds of oxidative DNA damage and start the way of DNA damage recovery. As a main DNA repairase,poly(ADP-ribose) polymerase (PARP)activation plays an important role in a number of pathological conditions associated with oxidative stress, such as cardiovascular and neurodegenerative diseases, cancer and inflammation, et al. Growing evidences indicate that PARP plays an important part in the pathogenesis of autoimmune diabetes mellitus as well as chronic complications and effects indispensablely.In the development of diabetic polyneuropathy(DPN), PARP activation is a obligatory initiator.Now we observe the effects of PARP inhibitor 3-aminobenzamide(3-AB)in streptozotocin-diabetic rat model of early peripheral neuropathy .Methods 30 male Wistar rats after being born 8 weeks,with body weights 150g～200g,were injected STZ( 60mg·kg-1) at left lower quadrant after fasting diet alterna nocte (STZ were dissolved in 0.1mol·L-1 natrium citricum, pH=4.5). Blood samples for measurements of glucose were taken from the tail veins approximately 72 h after STZ injection. The rats with blood glucose concentrations of 16.7 mmol/L or more were considered diabetic. Experimental rats were divided into control group( NC group,n=10),diabetic control group(DC group,n = 10)and diabetic group treated with 3-aminobenzamide(DT group,n=10).DT group were administrated with 3-AB every day oriented to time, the rest two groups were administrated with distilled water m.t.d for 2 weeks after 2 weeks. Nerve conduction velocity of the rats were assayed before the experiment was over. Executing the rats via decapitation ,the activity of serum SOD and the concentration of serum MDA,Pcr,cr which came from sciatic nerves were assayed after 4 weeks.Results 3-aminobenzamide enhanced the activity of serum SOD and Pcr of sciatic nerves in diabetic rats compared with controls (P<0.05), prevented sciatic nerve conduction velocity from decreasing(P<0.05),the concentration of serum MDA were decreased significantly(P<0.01) and the results of ATP and Cr in sciatic nerves were similar (P>0.05).Conclusion This short-term study with the PARP inhibitor: 3-aminobenzamide, saw the reversal of established functional and metabolic abnormalities of early DPN in the streptozotocin-diabetic rat model, provided a novel approach for the prevention and treatment of diabetic neuropathy.
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