Study On Quality Control And Intestinal Absorption Of The Total Flavonoid Of Fructus Aurantii

In this thesis, the method for the quality control of the effective fraction of Traditional Chinese Medicines was further developed. Moreover, the absorption characteristics and kinetics of the effective fraction were also studied. The research results will provide information for dosage form design and physiological disposition of the effective fraction.Based on index components content, overall composition content and finger-print analysis, an approach was proposed and applied to the quality control of the total flavonoids of Fructus Aurantii. The contents of naringin, neohesperidin and the total flavonoids, and fingerprinting analysis for different batches of the effective fractions of Fructus Aurantii. were investigated. The results showed that the contents of the index compounds and the total flavonoids in different batches of the effective fraction were fluctuated, but the similarities of their fingerprints were high conformity. It indicates that the proposed method can effectively control the quality of effective fractionThe intestinal absorption mechanism of naringin and neohesperidin was studied by the everted rat gut sacs. According to the absorption parameters, it was a first-order process with the passive diffusion mechanism in the range of the tested concentration, and could be absorbed in all segment of intestine in rat The absorption decreased with the raise of pH, and increased as verapamil existed. Other substances in the total flavonoids would promote the absorption of these two compounds.The absorption kinetics of the total flavonoids of Fructus Aurantii and the total glycosides of Radix Paeoniae Rubra was studied by in situ recirculation. as well as the difference between them when using co-administratively and exclusively. The results indicated that there was no significant difference of K_a's value of naringin, neohesperidin and paeoniflofin in different concentrations, their absorptions in rat intestinal were fit to a first-order process with the passive diffusion mechanism. No significant absorption difference was found between co-administration and exclusive situations too.